Genetic Variation among African Swine Fever Genotype II Viruses, Eastern and Central Europe
Carmina Gallardo
, Jovita Fernández-Pinero, Virginia Pelayo, Ismail Gazaev, Iwona Markowska-Daniel, Gediminas Pridotkas, Raquel Nieto, Paloma Fernández-Pacheco, Svetlana Bokhan, Oleg Nevolko, Zhanna Drozhzhe, Covadonga Pérez, Alejandro Soler, Denis Kolvasov, and Marisa Arias
Author affiliations: Centro de Investigación en Sanidad Animal (CISA-INIA), Madrid, Spain (C. Gallardo, J. Fernández-Pinero, V. Pelayo, R. Nieto, P. Fernández-Pacheco, C. Pérez, A. Soler, M. Arias); National Institute of Veterinary Virology and Microbiology, Pokrov, Russia (I. Gazaev, D. Kolvasov); National Veterinary Research Institute, Pulawy, Poland (I. Markowska-Daniel); National Food and Veterinary Risk Assessment Institute, Vilnius, Lithuania (G. Pridotkas); Belarusian State Veterinary Centre, Minsk, Belarus (S. Bokhan); State Research Institute of Laboratory Diagnostic and Veterinary Sanitary Expertise, Kiev, Ukraine (O. Nevolko, Z. Drozhzhe)
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Figure 2
Figure 2. Minimum evolution (ME) phylogenetic tree of African swine fever virus (ASFV) isolates from Lithuania and Poland based on the C-terminal end of the p72 coding gene relative to the 22 p72 genotypes (labeled I-XXII), including 88 nt sequences. The tree was inferred by using the ME method (http://www.megasoftware.net/mega4/WebHelp/part_iv___evolutionary_analysis/constructing_phylogenetic_trees/minimum_evolution_method/rh_minimum_evolution.htm) following initial application of a neighbor-joining algorithm. The phylogenetic tree was rooted by the midpoint method. The percentage of replicate trees >50% in which the associated taxa clustered together by bootstrap analysis (1,000 replicates) is shown adjacent to the nodes. The robustness of the ME tree was tested by using the close-neighbor-interchange algorithm at a search level of 1. Squares indicate ASFV isolates from Lithuania and Poland that were genotyped in this study; circles indicate ASFV isolates during 2007–2013 from the Caucasus region. Scale bar indicates nucleotide mutations per site.
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