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Volume 21, Number 10—October 2015

Methicillin-Susceptible, Vancomycin-Resistant Staphylococcus aureus, Brazil

Diana Panesso, Paul Planet, Lorena Diaz, Jean-Emmanuel Hugonnet, Truc T. Tran, Apurva Narechania, Jose M. Munita, Sandra Rincon, Lina P. Carvajal, Jinnethe Reyes, Alejandra Londoño, Hannah Smith, Robert Sebra, Gintaras Deikus, George M. Weinstock, Barbara E. Murray, Flavia Rossi, Michel Arthur, and Cesar A. AriasComments to Author 
Author affiliations: Universidad El Bosque, Bogota, Colombia (D. Panesso, P.J. Planet, L. Diaz, J.M. Munita, S. Rincon, L.P. Carvajal, J. Reyes, G. M. Weinstock, C.A. Arias); University of Texas Medical School, Houston, Texas, USA (D. Panesso, T.T. Tran, J.M. Munita, A. Londoño, B.E. Murray, C.A. Arias); American Museum of Natural History, New York (P.J. Planet, A. Narechania); Columbia University, New York, New York, USA (P.J. Planet, A. Londoño, H. Smith); Centre de Recherche des Cordeliers, Paris, France (J.E. Hugonnet, M. Arthur); Université Pierre et Marie Curie, Paris (J.E. Hugonnet, M. Arthur); Université Paris Descartes, Paris (J.E. Hugonnet, M. Arthur); Clinica Alemana de Santiago, Santiago, Chile (J.M. Munita); Universidad del Desarrollo, Santiago (J.M. Munita); Icahn School of Medicine at Mount Sinai, New York (R. Sebra, G. Deikus); The Jackson Laboratory for Genomic Medicine, Farmington, Connecticut, USA (G.M. Weinstock); Universidade da São Paulo, São Paulo, Brazil (F. Rossi)

Main Article

Table 1

Staphylococcus aureus strains used in analysis of methicillin and vancomycin resistance, Brazil*

Strain Strain characteristics MIC, μg/mL
Vancomycin Gentamicin
VS-MRSA Isolated from the bloodstream of a patient in Brazil 0.5 0.5 (1)
VR-MRSA Isolated from the blood of the same patient above and carrying vanA-containing pBRZ01 >256 32 (1)
VS-MSSA Isolated from the blood of the same patient 13 d after isolation of VR-MRSA 1 0.75 This study
VR-MSSA Isolated from the same blood culture as VS-MSSA 256 48 This study
RN4220-RF Laboratory strain of S.aureus used as recipient for mating experiments; fusidic acid and rifampin-resistant 1 1 (1)
Transconjugant 1† Transconjugant obtained from a mating experiment using VR-MSSA as donor and VS-MRSA as recipient >256 48 This study
Transconjugant 2† Transconjugant obtained from a mating experiment using VR-MRSA as donor and VS-MSSA as recipient >256 64 This study
Transconjugant 3† Transconjugant obtained from a mating experiment using VR-MSSA as donor and RN4220-RF as recipient >256 64 This study

*VS-MRSA, vancomycin-susceptible, methicillin-resistant S. aureus; VS-MSSA, vancomycin-susceptible, methicillin-susceptible S. aureus; VR-MSSA, vancomycin-resistant, methicillin-susceptible S. aureus.
†A fusidic acid–resistant derivative was generated for mating experiments. All mating experiments were performed on brain heart infusion (BHI) agar in the presence of vancomycin (32 µg/mL) and fusidic acid (25 µg/mL) to select for transconjugants.

Main Article

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Page updated: September 22, 2015
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