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Volume 21, Number 10—October 2015
Dispatch

Methicillin-Susceptible, Vancomycin-Resistant Staphylococcus aureus, Brazil

Diana Panesso, Paul Planet, Lorena Diaz, Jean-Emmanuel Hugonnet, Truc T. Tran, Apurva Narechania, Jose M. Munita, Sandra Rincon, Lina P. Carvajal, Jinnethe Reyes, Alejandra Londoño, Hannah Smith, Robert Sebra, Gintaras Deikus, George M. Weinstock, Barbara E. Murray, Flavia Rossi, Michel Arthur, and Cesar A. AriasComments to Author 
Author affiliations: Universidad El Bosque, Bogota, Colombia (D. Panesso, P.J. Planet, L. Diaz, J.M. Munita, S. Rincon, L.P. Carvajal, J. Reyes, G. M. Weinstock, C.A. Arias); University of Texas Medical School, Houston, Texas, USA (D. Panesso, T.T. Tran, J.M. Munita, A. Londoño, B.E. Murray, C.A. Arias); American Museum of Natural History, New York (P.J. Planet, A. Narechania); Columbia University, New York, New York, USA (P.J. Planet, A. Londoño, H. Smith); Centre de Recherche des Cordeliers, Paris, France (J.E. Hugonnet, M. Arthur); Université Pierre et Marie Curie, Paris (J.E. Hugonnet, M. Arthur); Université Paris Descartes, Paris (J.E. Hugonnet, M. Arthur); Clinica Alemana de Santiago, Santiago, Chile (J.M. Munita); Universidad del Desarrollo, Santiago (J.M. Munita); Icahn School of Medicine at Mount Sinai, New York (R. Sebra, G. Deikus); The Jackson Laboratory for Genomic Medicine, Farmington, Connecticut, USA (G.M. Weinstock); Universidade da São Paulo, São Paulo, Brazil (F. Rossi)

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Figure 1

Molecular typing of Staphylococcus aureus strains, Brazil. A) SmaI digestion of total DNA, followed by pulsed-field gel electrophoresis. Lane M, lambda ladder (molecular masses are indicated in kilobases on the left); lane 1, vancomycin-susceptible, methicillin-resistant S. aureus (VS-MRSA) isolated from the blood of a Brazilian patient (1); lane 2, vancomycin-resistant MRSA (VR-MRSA) isolated from the same patient and blood culture (1); lane 3, transconjugant 1 obtained from a mating experiment

Figure 1. Molecular typing of Staphylococcus aureus strains, Brazil. A) SmaI digestion of total DNA, followed by pulsed-field gel electrophoresis. Lane M, lambda ladder (molecular masses are indicated in kilobases on the left); lane 1, vancomycin-susceptible, methicillin-resistant S. aureus (VS-MRSA) isolated from the blood of a Brazilian patient (1); lane 2, vancomycin-resistant MRSA (VR-MRSA) isolated from the same patient and blood culture (1); lane 3, transconjugant 1 obtained from a mating experiment using vancomycin-resistant MSSA (VR-MSSA) as donor and VS-MRSA as recipient; lane 4, vancomycin-susceptible MSSA (VS-MSSA) isolated from the blood of the same patient 13 days after isolation of VR-MRSA; lane 5, VR-MSSA isolated at the same time as VS-MSSA; lane 6, transconjugant 2 obtained from a mating experiment using VR-MRSA as donor and VS-MSSA as recipient; lane 7, S. aureus RN4220 RF, lane 8, transconjugant 3 obtained using VR-MSSA as donor and RN4220 RF as recipient. B) S1 digestion of total DNA using the same strains shown in panel A. C) Hybridization with vanA probe using the same strains shown in panel A. Arrow indicates a positive signal for the vanA gene.

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