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Volume 21, Number 10—October 2015
Dispatch

Detection of Mixed Infections with Plasmodium spp. by PCR, India, 2014

Sri Krishna, Praveen K. Bharti, Himashu S. Chandel, Amreen Ahmad, Rajesh Kumar, Puspendra P. Singh, Mrigendra P. Singh, and Neeru SinghComments to Author 
Author affiliations: National Institute for Research in Tribal Health, Jabalpur, India (S. Krishna, P.K. Bharti, H.S. Chandel, A. Ahmad, R. Kumar, P.P. Singh, N. Singh); National Institute for Malaria Research Field Unit, Jabalpur (M.P. Singh)

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Figure 2

Identification of Plasmodium spp. by nested PCR at 15 community health centers in 8 states in India to which malaria is endemic. A) Plasmodium falciparum (205-bp fragment). Lane 1, molecular mass marker; lane 2, negative (–) control; lane 3, positive (+) control; lanes 7−27, positive samples; lanes 5 and 6, negative samples. B) P. malariae (144-bp fragment). Lane 25, + control; lane 26, – control; lane 27, molecular mass marker; lane 12, positive sample; lanes 1–11, 13–24, negative samples. C) P

Figure 2. Identification of Plasmodium spp. by nested PCR at 15 community health centers in 8 states in India to which malaria is endemic. A) Plasmodium falciparum (205-bp fragment). Lane 1, molecular mass marker; lane 2, negative (–) control; lane 3, positive (+) control; lanes 7−27, positive samples; lanes 5 and 6, negative samples. B) P. malariae (144-bp fragment). Lane 25, + control; lane 26, – control; lane 27, molecular mass marker; lane 12, positive sample; lanes 1–11, 13–24, negative samples. C) P. ovale (800-bp fragment). Lane 1, molecular mass marker; lane 2, – control; lane 3, + control; lane 17, positive sample; lanes 4–16, 18–27, negative samples. D) P. vivax (120-bp fragment). Lane 1, molecular mass marker; lane 2, + control; lane 3, – control; lanes 5, 11, 16, and 25, positive samples; lanes 4, 6–10, 12–15, 17–24, 26, and 27, negative samples.

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Page updated: September 22, 2015
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