Volume 21, Number 2—February 2015
Online Report
Melioidosis Diagnostic Workshop, 20131
Figure

Figure. Diagnostic guidelines for clinicians and microbiologists in developed countries and resource-limited settings. 1) The antimicrobial drug–susceptibility pattern can be useful for distinguishing Burkholderia pseudomallei (usually resistant to aminoglycosides and colistin or polymyxin but susceptible to amoxicillin/clavulanic acid) from other pathogenic species. However, isolates can occasionally be susceptible to aminoglycosides; susceptibility may vary by region (40). If disk diffusion is used, zone diameter interpretation may need to be modified from break points recommended for Enterobacteriaceae by the Clinical and Laboratory Standards Institute (73). 2) Not currently commercially available. API 20NE, bioMérieux, Craponne, France; VITEK, Becton, Dickinson, and Company, Franklin Lakes, NJ, USA.
References
- Wiersinga WJ, Currie BJ, Peacock SJ. Melioidosis. N Engl J Med. 2012;367:1035–44. DOIPubMedGoogle Scholar
- Ngauy V, Lemeshev Y, Sadkowski L, Crawford G. Cutaneous melioidosis in a man who was taken as a prisoner of war by the Japanese during World War II. J Clin Microbiol. 2005;43:970–2. DOIPubMedGoogle Scholar
- Peacock SJ, Schweizer HP, Dance DA, Smith TL, Gee JE, Wuthiekanun V, Management of accidental laboratory exposure to Burkholderia pseudomallei and B. mallei. Emerg Infect Dis. 2008;14:e2. DOIPubMedGoogle Scholar
- John TJ, Jesudason MV, Lalitha MK, Ganesh A, Mohandas V, Cherian T, Melioidosis in India: the tip of the iceberg? Indian J Med Res. 1996;103:62–5 .PubMedGoogle Scholar
- Kibbler CC, Roberts CM, Ridgway GL, Spiro SG. Melioidosis in a patient from Bangladesh. Postgrad Med J. 1991;67:764–6. DOIPubMedGoogle Scholar
- Stewart T, Engelthaler DM, Blaney DD, Tuanyok A, Wangsness E, Smith TL, Epidemiology and investigation of melioidosis, southern Arizona. Emerg Infect Dis. 2011;17:1286–8. DOIPubMedGoogle Scholar
- Zehnder AM, Hawkins MG, Koski MA, Lifland B, Byrne BA, Swanson AA, Burkholderia pseudomallei isolates in 2 pet iguanas, California, USA. Emerg Infect Dis. 2014;20:304–6. DOIPubMedGoogle Scholar
- Wuthiekanun V, Dance DA, Wattanagoon Y, Supputtamongkol Y, Chaowagul W, White NJ. The use of selective media for the isolation of Pseudomonas pseudomallei in clinical practice. J Med Microbiol. 1990;33:121–6. DOIPubMedGoogle Scholar
- Wuthiekanun V, Suputtamongkol Y, Simpson AJ, Kanaphun P, White NJ. Value of throat swab in diagnosis of melioidosis. J Clin Microbiol. 2001;39:3801–2. DOIPubMedGoogle Scholar
- Cheng AC, Wuthiekanun V, Limmathurosakul D, Wongsuvan G, Day NP, Peacock SJ. Role of selective and nonselective media for isolation of Burkholderia pseudomallei from throat swabs of patients with melioidosis. J Clin Microbiol. 2006;44:2316. DOIPubMedGoogle Scholar
- Peacock SJ, Chieng G, Cheng AC, Dance DA, Amornchai P, Wongsuvan G, Comparison of Ashdown’s medium, Burkholderia cepacia medium, and Burkholderia pseudomallei selective agar for clinical isolation of Burkholderia pseudomallei. J Clin Microbiol. 2005;43:5359–61 and. DOIPubMedGoogle Scholar
- Thorn P, Krause V, Boutlis C, Currie B. Melioidosis in the Top End. The Northern Territory Disease Control Bulletin. 2005;12:1–2 [cited 2014 Dec 4]. http://www.health.nt.gov.au/library/scripts/objectifyMedia.aspx?file=pdf/11/41.pdf&siteID=1&str_title=Bulletin+December+2005.pdf
- Limmathurotsakul D, Wuthiekanun V, Chierakul W, Cheng AC, Maharjan B, Chaowagul W, Role and significance of quantitative urine cultures in diagnosis of melioidosis. J Clin Microbiol. 2005;43:2274–6. DOIPubMedGoogle Scholar
- Centers for Disease Control and Prevention. Laboratory exposure to Burkholderia pseudomallei—Los Angeles, California, 2003. MMWR Morb Mortal Wkly Rep. 2004;53:988–90 .PubMedGoogle Scholar
- Pumpuang A, Chantratita N, Wikraiphat C, Saiprom N, Day NP, Peacock SJ, Survival of Burkholderia pseudomallei in distilled water for 16 years. Trans R Soc Trop Med Hyg. 2011;105:598–600. DOIPubMedGoogle Scholar
- Yabuuchi E, Wang L, Arakawa M, Yano I. Survival of Pseudomonas pseudomallei strains at 5 degrees C. Kansenshogaku Zasshi. 1993;67:331–5. DOIPubMedGoogle Scholar
- Larsen E, Smith JJ, Norton R, Corkeron M. Survival, sublethal injury, and recovery of environmental Burkholderia pseudomallei in soil subjected to desiccation. Appl Environ Microbiol. 2013;79:2424–7. DOIPubMedGoogle Scholar
- Holland DJ, Wesley A, Drinkovic D, Currie BJ. Cystic fibrosis and Burkholderia pseudomallei: an emerging problem? Clin Infect Dis. 2002;35:e138–40. DOIPubMedGoogle Scholar
- Wesley A, Holland D, Currie B. Burkholderia pseudomallei in two siblings with cystic fibrosis and evidence of likely person to person transmission in the family setting. In: Proceedings of Fourth Australian and New Zealand Cystic Fibrosis Conference; 2001 Aug 23–25, Brisbane, Australia. p. 46.
- Price EP, Sarovich DS, Mayo M, Tuanyok A, Drees KP, Kaestli M, Within-host evolution of Burkholderia pseudomallei over a twelve-year chronic carriage infection. MBiol. 2013;4:e00388–13.PubMedGoogle Scholar
- O’Sullivan BP, Torres B, Conidi G, Smole S, Gauthier C, Stauffer KE, Burkholderia pseudomallei infection in a child with cystic fibrosis: acquisition in the Western Hemisphere. Chest. 2011;140:239–42. DOIPubMedGoogle Scholar
- Huis in ’t Veld D, Wuthiekanun V, Cheng AC, Chierakul W, Chaowagul W, Brouwer AE, The role and significance of sputum cultures in the diagnosis of melioidosis. Am J Trop Med Hyg. 2005;73:657–61 .PubMedGoogle Scholar
- Limmathurotsakul D, Jamsen K, Arayawichanont A, Simpson JA, White LJ, Lee SJ, Defining the true sensitivity of culture for the diagnosis of melioidosis using Bayesian latent class models. PLoS ONE. 2010;5:e12485. DOIPubMedGoogle Scholar
- Roesnita B, Tay ST, Puthucheary SD, Sam IC. Diagnostic use of Burkholderia pseudomallei selective media in a low prevalence setting. Trans R Soc Trop Med Hyg. 2012;106:131–3. DOIPubMedGoogle Scholar
- Glass MB, Beesley CA, Wilkins PP, Hoffmaster AR. Comparison of four selective media for the isolation of Burkholderia mallei and Burkholderia pseudomallei. Am J Trop Med Hyg. 2009;80:1023–8 .PubMedGoogle Scholar
- Wongsuvan G, Limmathurotsakul D, Wannapasni S, Chierakul W, Teerawattanasook N, Wuthiekanun V. Lack of correlation of Burkholderia pseudomallei quantities in blood, urine, sputum and pus. Southeast Asian J Trop Med Public Health. 2009;40:781–4 .PubMedGoogle Scholar
- Dance DA, Wuthiekanun V, Naigowit P, White NJ. Identification of Pseudomonas pseudomallei in clinical practice: use of simple screening tests and API 20NE. J Clin Pathol. 1989;42:645–8. DOIPubMedGoogle Scholar
- Tandhavanant S, Wongsuvan G, Wuthiekanun V, Teerawattanasook N, Day NP, Limmathurotsakul D, Monoclonal antibody-based immunofluorescence microscopy for the rapid identification of Burkholderia pseudomallei in clinical specimens. Am J Trop Med Hyg. 2013;89:165–8. DOIPubMedGoogle Scholar
- Inglis TJ, Chiang D, Lee GS, Chor-Kiang L. Potential misidentification of Burkholderia pseudomallei by API 20NE. Pathology. 1998;30:62–4. DOIPubMedGoogle Scholar
- Lowe P, Engler C, Norton R. Comparison of automated and nonautomated systems for identification of Burkholderia pseudomallei. J Clin Microbiol. 2002;40:4625–7. DOIPubMedGoogle Scholar
- Inglis TJ, Merritt A, Chidlow G, Aravena-Roman M, Harnett G. Comparison of diagnostic laboratory methods for identification of Burkholderia pseudomallei. J Clin Microbiol. 2005;43:2201–6. DOIPubMedGoogle Scholar
- Glass MB, Popovic T. Preliminary evaluation of the API 20NE and RapID NF plus systems for rapid identification of Burkholderia pseudomallei and B. mallei. J Clin Microbiol. 2005;43:479–83. DOIPubMedGoogle Scholar
- Amornchai P, Chierakul W, Wuthiekanun V, Mahakhunkijcharoen Y, Phetsouvanh R, Currie BJ, Accuracy of Burkholderia pseudomallei identification using the API 20NE system and a latex agglutination test. J Clin Microbiol. 2007;45:3774–6. DOIPubMedGoogle Scholar
- Koh TH, Yong Ng LS, Foon Ho JL, Sng LH, Wang GC, Tzer Pin Lin RV. Automated identification systems and Burkholderia pseudomallei. J Clin Microbiol. 2003;41:1809. DOIPubMedGoogle Scholar
- Weissert C, Dollenmaier G, Rafeiner P, Riehm J, Schultze D. Burkholderia pseudomallei misidentified by automated system. Emerg Infect Dis. 2009;15:1799–801. DOIPubMedGoogle Scholar
- Lowe P, Haswell H, Lewis K. Use of various common isolation media to evaluate the new VITEK 2 colorimetric GN card for identification of Burkholderia pseudomallei. J Clin Microbiol. 2006;44:854–6. DOIPubMedGoogle Scholar
- Zong Z, Wang X, Deng Y, Zhou T. Misidentification of Burkholderia pseudomallei as Burkholderia cepacia by the VITEK 2 system. J Med Microbiol. 2012;61:1483–4. DOIPubMedGoogle Scholar
- Podin Y, Kaestli M, McMahon N, Hennessy J, Ngian HU, Wong JS, Reliability of automated biochemical identification of Burkholderia pseudomallei is regionally dependent. J Clin Microbiol. 2013;51:3076–8. DOIPubMedGoogle Scholar
- Hodgson K, Engler C, Govan B, Ketheesan N, Norton R. Comparison of routine bench and molecular diagnostic methods in identification of Burkholderia pseudomallei. J Clin Microbiol. 2009;47:1578–80. DOIPubMedGoogle Scholar
- Podin Y, Sarovich DS, Price EP, Kaestli M, Mayo M, Hii K, Burkholderia pseudomallei isolates from Sarawak, Malaysian Borneo, are predominantly susceptible to aminoglycosides and macrolides. Antimicrob Agents Chemother. 2014;58:162–6. DOIPubMedGoogle Scholar
- Anuntagool N, Naigowit P, Petkanchanapong V, Aramsri P, Panichakul T, Sirisinha S. Monoclonal antibody-based rapid identification of Burkholderia pseudomallei in blood culture fluid from patients with community-acquired septicaemia. J Med Microbiol. 2000;49:1075–8 .PubMedGoogle Scholar
- Dharakul T, Songsivilai S, Smithikarn S, Thepthai C, Leelaporn A. Rapid identification of Burkholderia pseudomallei in blood cultures by latex agglutination using lipopolysaccharide-specific monoclonal antibody. Am J Trop Med Hyg. 1999;61:658–62 .PubMedGoogle Scholar
- Ekpo P, Rungpanich U, Pongsunk S, Naigowit P, Petkanchanapong V. Use of protein-specific monoclonal antibody-based latex agglutination for rapid diagnosis of Burkholderia pseudomallei infection in patients with community-acquired septicemia. Clin Vaccine Immunol. 2007;14:811–2. DOIPubMedGoogle Scholar
- Kiratisin P, Santanirand P, Chantratita N, Kaewdaeng S. Accuracy of commercial systems for identification of Burkholderia pseudomallei versus Burkholderia cepacia. Diagn Microbiol Infect Dis. 2007;59:277–81 . DOIPubMedGoogle Scholar
- Lowe W, March JK, Bunnell AJ, O’Neill KL, Robison RA. PCR-based methodologies used to detect and differentiate the Burkholderia pseudomallei complex: B. pseudomallei, B. mallei, and B. thailandensis. Curr Issues Mol Biol. 2013;16:23–54 .PubMedGoogle Scholar
- Price EP, Dale JL, Cook JM, Sarovich DS, Seymour ML, Ginther JL, Development and validation of Burkholderia pseudomallei–specific real-time PCR assays for clinical, environmental or forensic detection applications. PLoS ONE. 2012;7:e37723. DOIPubMedGoogle Scholar
- Novak RT, Glass MB, Gee JE, Gal D, Mayo MJ, Currie BJ, Development and evaluation of a real-time PCR assay targeting the type III secretion system of Burkholderia pseudomallei. J Clin Microbiol. 2006;44:85–90. DOIPubMedGoogle Scholar
- Morse SA, Kellogg RB, Perry S, Meyer RF, Bray D, Nichelson D, Detecting biothreat agents: the Laboratory Response Network. ASM News. 2003;69:433–7.
- Gee JE, Sacchi CT, Glass MB, De BK, Weyant RS, Levett PN, Use of 16S rRNA gene sequencing for rapid identification and differentiation of Burkholderia pseudomallei and B. mallei. J Clin Microbiol. 2003;41:4647–54 . DOIPubMedGoogle Scholar
- Clinical and Laboratory Standards Institute. Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria. CLSI document M45–A2. Wayne (PA): The Institute; 2010.
- Wuthiekanun V, Cheng AC, Chierakul W, Amornchai P, Limmathurotsakul D, Chaowagul W, Trimethoprim/sulfamethoxazole resistance in clinical isolates of Burkholderia pseudomallei. J Antimicrob Chemother. 2005;55:1029–31. DOIPubMedGoogle Scholar
- Jenney AW, Lum G, Fisher DA, Currie BJ. Antibiotic susceptibility of Burkholderia pseudomallei from tropical northern Australia and implications for therapy of melioidosis. Int J Antimicrob Agents. 2001;17:109–13. DOIPubMedGoogle Scholar
- Piliouras P, Ulett GC, Ashhurst-Smith C, Hirst RG, Norton RE. A comparison of antibiotic susceptibility testing methods for cotrimoxazole with Burkholderia pseudomallei. Int J Antimicrob Agents. 2002;19:427–9. DOIPubMedGoogle Scholar
- Chantratita N, Tandhavanant S, Wongsuvan G, Wuthiekanun V, Teerawattanasook N, Day NP, Rapid detection of Burkholderia pseudomallei in blood cultures using a monoclonal antibody–based immunofluorescent assay. Am J Trop Med Hyg. 2013;89:971–2. DOIPubMedGoogle Scholar
- Wuthiekanun V, Desakorn V, Wongsuvan G, Amornchai P, Cheng AC, Maharjan B, Rapid immunofluorescence microscopy for diagnosis of melioidosis. Clin Diagn Lab Immunol. 2005;12:555–6 .PubMedGoogle Scholar
- Thibault FM, Valade E, Vidal DR. Identification and discrimination of Burkholderia pseudomallei, B. mallei, and B. thailandensis by real-time PCR targeting type III secretion system genes. J Clin Microbiol. 2004;42:5871–4 . DOIPubMedGoogle Scholar
- Kaestli M, Richardson LJ, Colman RE, Tuanyok A, Price EP, Bowers JR, Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens. J Clin Microbiol. 2012;50:2059–62. DOIPubMedGoogle Scholar
- Meumann EM, Novak RT, Gal D, Kaestli ME, Mayo M, Hanson JP, Clinical evaluation of a type III secretion system real-time PCR assay for diagnosing melioidosis. J Clin Microbiol. 2006;44:3028–30. DOIPubMedGoogle Scholar
- Chantratita N, Meumann E, Thanwisai A, Limmathurotsakul D, Wuthiekanun V, Wannapasni S, Loop-mediated isothermal amplification method targeting the TTS1 gene cluster for the detection of Burkholderia pseudomallei and diagnosis of melioidosis. J Clin Microbiol. 2008;46:568–73 . DOIPubMedGoogle Scholar
- Houghton RL, Reed DE, Hubbard MA, Dillon MJ, Chen H, Currie BJ, Development of a prototype lateral flow immunoassay (LFI) for the rapid diagnosis of melioidosis. PLoS Negl Trop Dis. 2014;8:e2727. DOIPubMedGoogle Scholar
- Sim BM, Chantratita N, Ooi WF, Nandi T, Tewhey R, Wuthiekanun V, Genomic acquisition of a capsular polysaccharide virulence cluster by non-pathogenic Burkholderia isolates. Genome Biol. 2010;11:R89. DOIPubMedGoogle Scholar
- Cheng AC, O’Brien M, Freeman K, Lum G, Currie BJ. Indirect hemagglutination assay in patients with melioidosis in northern Australia. Am J Trop Med Hyg. 2006;74:330–4 .PubMedGoogle Scholar
- Cheng AC, Peacock SJ, Limmathurotsakul D, Wongsuvan G, Chierakul W, Amornchai P, Prospective evaluation of a rapid immunochromogenic cassette test for the diagnosis of melioidosis in northeast Thailand. Trans R Soc Trop Med Hyg. 2006;100:64–7. DOIPubMedGoogle Scholar
- Harris PN, Ketheesan N, Owens L, Norton RE. Clinical features that affect indirect-hemagglutination-assay responses to Burkholderia pseudomallei. Clin Vaccine Immunol. 2009;16:924–30. DOIPubMedGoogle Scholar
- Armstrong PK, Anstey NM, Kelly PM, Currie BJ, Martins N, Dasari P, Seroprevalence of Burkholderia pseudomallei in East Timorese refugees: implications for healthcare in East Timor. Southeast Asian J Trop Med Public Health. 2005;36:1496–502 .PubMedGoogle Scholar
- Clayton AJ, Lisella RS, Martin DG. Melioidosis: a serological survey in military personnel. Mil Med. 1973;138:24–6 .PubMedGoogle Scholar
- Chantratita N, Wuthiekanun V, Thanwisai A, Limmathurotsakul D, Cheng AC, Chierakul W, Accuracy of enzyme-linked immunosorbent assay using crude and purified antigens for serodiagnosis of melioidosis. Clin Vaccine Immunol. 2007;14:110–3. DOIPubMedGoogle Scholar
- Limmathurotsakul D, Chantratita N, Teerawattanasook N, Piriyagitpaiboon K, Thanwisai A, Wuthiekanun V, Enzyme-linked immunosorbent assay for the diagnosis of melioidosis: better than we thought. Clin Infect Dis. 2011;52:1024–8. DOIPubMedGoogle Scholar
- Trikalinos TA, Balion CM. Chapter 9: options for summarizing medical test performance in the absence of a “gold standard.”. J Gen Intern Med. 2012;27(Suppl 1):S67–75. DOIPubMedGoogle Scholar
- Lim C, Wannapinij P, White L, Day NP, Cooper BS, Peacock SJ, Using a web-based application to define the accuracy of diagnostic tests when the gold standard is imperfect. PLoS ONE. 2013;8:e79489. DOIPubMedGoogle Scholar
- Karger A, Stock R, Ziller M, Elschner MC, Bettin B, Melzer F, Rapid identification of Burkholderia mallei and Burkholderia pseudomallei by intact cell matrix-assisted laser desorption/ionisation mass spectrometric typing. BMC Microbiol. 2012;12:229. DOIPubMedGoogle Scholar
- Weigel LM, Sue D, Michel PA, Kitchel B, Pillai SP. A rapid antimicrobial susceptibility test for Bacillus anthracis. Antimicrob Agents Chemother. 2010;54:2793–800 . DOIPubMedGoogle Scholar
- Chetchotisakd P, Chierakul W, Chaowagul W, Anunnatsiri S, Phimda K, Mootsikapun P, Trimethoprim-sulfamethoxazole versus trimethoprim-sulfamethoxazole plus doxycycline as oral eradicative treatment for melioidosis (MERTH): a multicentre, double-blind, non-inferiority, randomised controlled trial. Lancet. 2014;383:807–14 . DOIPubMedGoogle Scholar
1Held September 14, 2013, in Bangkok, Thailand.