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Volume 21, Number 2—February 2015
Online Report

Melioidosis Diagnostic Workshop, 20131

Alex R. HoffmasterComments to Author , David AuCoin, Prasith Baccam, Henry C. Baggett, Rob Baird, Saithip Bhengsri, David D. Blaney, Paul J. Brett, Timothy J.G. Brooks, Katherine A. Brown, Narisara Chantratita, Allen C. Cheng, David A.B. Dance, Saskia Decuypere, Dawn Defenbaugh, Jay E. Gee, Raymond Houghton, Possawat Jorakate, Ganjana Lertmemongkolchai, Direk Limmathurotsakul, Toby L. Merlin, Chiranjay Mukhopadhyay, Robert Norton, Sharon J. Peacock, Dionne B. Rolim, Andrew J. Simpson, Ivo Steinmetz, Robyn A. Stoddard, Martha M. Stokes, David Sue, Apichai Tuanyok, Toni Whistler, Vanaporn Wuthiekanun, and Henry Walke
Author affiliations: US Centers for Disease Control and Prevention, Atlanta, Georgia, USA (A.R. Hoffmaster, D.D. Blaney, J.E. Gee, T.L. Merlin, R.A. Stoddard, D. Sue, H.T. Walke); University of Nevada School of Medicine, Reno, Nevada, USA (D. AuCoin); IEM, Research Triangle Park, North Carolina, USA (P. Baccam); Royal Darwin Hospital, Darwin, Northern Territory, Australia (R. Baird); US Centers for Disease Control and Prevention, Nonthaburi, Thailand (H.C. Baggett, S. Bhengsri, P. Jorakate, T. Whistler); University of South Alabama, Mobile, Alabama, USA (P.J. Brett); Public Health England, Salisbury, UK (T.J.G. Brooks, A.J. Simpson); University of Texas, Austin, Texas, USA (K.A. Brown); University of Cambridge, Cambridge, UK (K.A. Brown, S.J. Peacock); Mahidol University, Bangkok, Thailand (N. Chantratita, D. Limmathurotsakul, V. Wuthiekanun); Alfred Health, Monash University, Melbourne, Victoria, Australia (A. C. Cheng); Lao-Oxford-Mahosot Hospital–Wellcome Trust Research Unit, Vientiane, Laos, and University of Oxford, Oxford, UK (D.A.B. Dance); University of Western Australia, Perth, Western Australia, Australia (S. Decuypere); Defense Threat Reduction Agency, Fort Belvoir, Virginia, USA (D. Defenbaugh, M.M. Stokes); InBios International, Seattle, Washington, USA (R. Houghton); Khon Kaen University, Khon Kaen, Thailand (G. Lertmemogkolchai); Kasturba Medical College, Manipal, India (C. Mukhopadhyay); Townsville Hospital, Townsville, Queensland, Australia (R. Norton); Universidade de Fortaleza, Fortaleza, Brazil (D. B. Rolim); University of Greifswald, Greifswald, Germany (I. Steinmetz); University of Hawaii at Manoa, Honolulu, Hawaii, USA (A. Tuanyok)

Main Article

Table 1

Performance of commercially available systems for identification of Burkholderia pseudomallei

Method Isolate source B. pseudomallei, no. correct/no. tested (%) Reference
API 20 NE* Thailand 390/400 (98) (27)
API 20 NE Singapore 40/50 (80) (29)
API 20 NE Australia 101/103 (98) (30)
API 20 NE Australia 26/71 (37) (31)
API 20 NE United States (imported) 35/58 (60) (32)
API 20 NE Thailand/various 792/800 (99) (33)
Phoenix† Singapore 13/47 (28) (34)
Phoenix Malaysia/Thailand 0/1 (0) (35)
VITEK 2* Australia 19/103 (19) (30)
VITEK 2 Australia 83/103 (81) (36)
VITEK 2 Malaysia 0/1 (0) (37)
VITEK 2 Australia 146/149 (98) (38)
VITEK 2 Sabah, Malaysia 22/25 (88) (38)
VITEK 2 Sarawak, Malaysia 23/43 (53) (38)

*bioMérieux, Craponne, France.
†Becton, Dickinson, and Company, Franklin Lakes, NJ, USA.

Main Article

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Main Article

1Held September 14, 2013, in Bangkok, Thailand.

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