Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 21, Number 3—March 2015
Dispatch

Comparison of Porcine Epidemic Diarrhea Viruses from Germany and the United States, 2014

Dennis Hanke1, Maria Jenckel1, Anja Petrov, Mathias Ritzmann, Julia Stadler, Valerij Akimkin, Sandra BlomeComments to Author , Anne Pohlmann, Horst Schirrmeier, Martin Beer, and Dirk Höper
Author affiliations: Friedrich-Loeffler-Institut, Greifswald–Insel Riems, Germany (D. Hanke, M. Jenckel, A. Petrov, S. Blome, A. Pohlmann, H. Schirrmeier, M. Beer, D. Höper); Ludwig-Maximilians-University Munich, Oberschleissheim, Germany (M. Ritzmann, J. Stadler); Chemical and Veterinary Investigations Office Stuttgart, Fellbach, Germany (V. Akimkin)

Main Article

Table

Sequencing results of porcine epidemic diarrhea virus isolated from pigs in Germany, 2014*

Isolate Mapped reads Average depth Total length GenBank accession no. Variant position
(full genome) Variant
position
(in CDS) Codon
change Amino acid substitution Variant frequency, %
L00719
 30,723
 146
 28,028
 LM645058
 19,334
 G19042U
polyprotein
 agu→auu
 S6348I
 48
20,880 U247G
S protein ucu→gcu S83A† 41
21,017 U384A
S protein aau→aaa N128K‡ 43
21,232 C599U
S protein gcu→guu A200V§ 14
21,328 G695U
S protein agu→auu S232I¶ 41
22,527 C1894U
S protein cuu→uuu L632F# 13
22,844 C2211U
S protein gac→gat Silent 46
22,970
 U2337C
S protein
 auu→auc
 Silent
 44
L00721 19,274 104 28,028 LM645057 None None None None None

*CDS, coding sequences; S protein, spike protein. Blank cells indicate not applicable.
†aa 83A also found in strain OH851 (GenBank accession no. KJ399978).
‡aa 128K found in all compared reference sequences.
§aa 200V not detected in reference sequences.
¶aa 232I detected in recent highly virulent strains (GenBank accession nos. KJ778615, KF272920, KF468753, KJ584361, KJ408801, KF650374, KF468752, KF267450, KC210147, JN825712, KC210145).
#aa 632F detected in reference strain KF267450 only.

Main Article

1These authors contributed equally to this article.

Page created: February 18, 2015
Page updated: February 18, 2015
Page reviewed: February 18, 2015
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external