Volume 21, Number 4—April 2015
Candidate New Rotavirus Species in Sheltered Dogs, Hungary
|Mapped read count||Average coverage (X)||Mapped read count||Average coverage (X)|
*Total sequence reads to obtain genomic RNA sequence for KE135/2012 and KE 528/2012 were 1,591,803, and 144,747, respectively. The minimum overlap with the consensus sequences (i.e., the de novo assembled rotavirus I–specific consensus sequences) was 20 nt, the minimum identity was 80%. To improve the mapping results, the following gap penalties were applied for the dataset: mismatch cost = 2, insertion cost = 3, deletion cost = 3. After visual inspection of the sequence alignments and remapping onto the obtained gene sequence, a single consensus sequence was finalized for each genome segment.