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Volume 21, Number 4—April 2015
Dispatch

Pathogenicity of 2 Porcine Deltacoronavirus Strains in Gnotobiotic Pigs

Kwonil JungComments to Author , Hui Hu, Bryan Eyerly, Zhongyan Lu, Juliet Chepngeno, and Linda J. SaifComments to Author 
Author affiliations: Ohio State University, Wooster, Ohio, USA

Main Article

Table 2

Histopathologic findings after inoculation of gnotobiotic pigs with PDCoV strains OH-FD22 and OH-FD100*

Pig status, no. hpi at euthanasia Mean VH:CD RNA detection in formalin-fixed, paraffin-embedded tissues/antigen detection in frozen tissues (ISH/IF results)†
Duodenum Jejunum Ileum Cecum/colon
PDCoV-inoculated
1 120 ND −/− +/+++ +/+++ –/±
2‡ NA NA NA NA NA NA
3 72 3.6 (1.7) –/± ++/+++ ++/+++ ±/±
4 96 1.4 (0.1) −/− ++/+++ ++/+++ –/±
5 72 1.6 (0.5) –/+ ++/+++ ++/+++ –/±
Negative control
6 NA 5.8 (0.9) −/− −/− −/− −/−
7 NA 5.6 (0.5) −/− −/− −/− −/−

*PDCoV, porcine deltacoronavirus; hpi, hour postinoculation; VH:CD, ratio of villous height to crypt depth; ISH, in situ hybridization; IF, immunofluorescence staining; ND, not determined because of autolysis at tips of jejunal villi; NA, not applicable.
†Detected by ISH or IF staining. –, no cells showed staining; +, 1%–29% of epithelial cells showed staining; +++, 60%–100% of epithelial cells showed staining as described (12); ±, <1% of epithelial cells showed staining; ++, 30%–59% of epithelial cells showed staining. Other internal organs showed no ISH or IF staining.
‡Pig 2 was used for production of hyperimmune gnotobiotic antiserum against PDCoV strain OH-FD22.

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Page updated: March 17, 2015
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