Volume 21, Number 4—April 2015
Dispatch
Pathogenicity of 2 Porcine Deltacoronavirus Strains in Gnotobiotic Pigs
Kwonil Jung
, Hui Hu, Bryan Eyerly, Zhongyan Lu, Juliet Chepngeno, and Linda J. Saif
, Hui Hu, Bryan Eyerly, Zhongyan Lu, Juliet Chepngeno, and Linda J. Saif
Table 1
Fecal shedding of virus and clinical signs after inoculation of gnotobiotic pigs with PDCoV strains OH-FD22 and OH-FD100*
| Pig status, no. (age at inoculation, d) | Virus strain |
Oral inoculum, log10 GEs |
Fecal shedding, log10 GE/mL by hpi† | Clinical signs (onset hpi) |
|||||
|---|---|---|---|---|---|---|---|---|---|
| 0 |
24 |
48 |
72 |
96 |
120 |
||||
| PDCoV-inoculated | |||||||||
| 1 (14) | OH-FD22 | 8.8 | <4.6 | <4.6 | 8.1 | 7.3 | 7.4 | 6.7‡ | Diarrhea/vomiting (21–24)§ |
| 2 (14) | OH-FD22 | 8.8 | <4.6 | 6.2 | 8.5 | 8.4 | 6.1 | 7.6 | Diarrhea/vomiting (21–24) |
| 3 (14) | OH-FD22 | 8.8 | <4.6 | 8.4 | 8.2 | 8.8‡ | – | – | Diarrhea/vomiting (21–24) |
| 4 (11) | OH-FD100 | 11.0 | <4.6 | 7.1 | 6.0 | ND | ND‡ | – | Diarrhea/vomiting (22–24) |
| 5 (11) | OH-FD100 | 11.0 | <4.6 | 8.2 | 8.5 | ND‡ | – | – | Diarrhea/vomiting (22–24) |
| Negative control | |||||||||
| 6 (16)¶ | None | None | <4.6 | <4.6 | <4.6 | <4.6‡ | – | – | None |
| 7 (17)¶ | None | None | <4.6 | <4.6 | <4.6 | <4.6‡ | – | – | None |
*PDCoV, porcine deltacoronavirus; GE, genome equivalent; hpi, hour postinoculation; –, no result (pig euthanized); ND, not determined.
†Detected by real-time quantitative reverse transcription PCR. The detection limit of the PCR was <4.6 log10 GE/mL for a fecal sample and <3.6 log10 GE/mL for a serum sample.
‡Pig was euthanized.
§Pig 1 vomited at days postinoculation 21–24 and then had diarrhea.
¶At euthanasia.
Page created: March 17, 2015
Page updated: March 17, 2015
Page reviewed: March 17, 2015
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