Volume 22, Number 12—December 2016
Dispatch
Horizontal Transmission of Chronic Wasting Disease in Reindeer
Table 2
Resutls of tissue testing for chronic wasting disease in reindeer based on immunohistochemical detection of PrPSc, assessment of spongiform change in formalin-fixed tissues, or both*
| Group no./animal no. | Survival time,¶ mpi | Clinical features | CNS tissues† |
Retina‡ | Other tissues§ |
||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Spinal cord | Obex | Cerebellum | Midbrain | Thalamus | Neocortex | Lymphoid head | Lymphoid other | Rectal mucosa | Pituitary | Spleen | Intestines | Skeletal muscle | Heart | Peripheral nervous system | Kidney | Gut tube | Adrenal | ||||
| 1 | |||||||||||||||||||||
| 1 | 2.6# | FD | NA/NA | −/− | −/− | −/− | −/− | −/− | NA | − | − | NA | NA | NA | NA | NA | NA | NA | − | NA | − |
| 2 | 20.9 | LBC, FD | +/+ | +/+ | −/− | −/+ | +/+ | +/+ | + | + | + | − | + | + | + | − | − | − | + | + | + |
| 3 | 33.9 | FD | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | + | + | + | + | + | + | + | + | − | − | − | − | |
| 4 | 34.1 | Seizures | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | + | + | + | + | + | + | − | − | − | + | − | − | − |
| 5 |
53.3 |
FD |
−/+ |
+/+ |
−/+ |
+/+ |
+/+ |
−/− |
+ |
+ |
+ |
+ |
+ |
NA |
− |
NA |
NA |
− |
NA |
NA |
NA |
| 2 | |||||||||||||||||||||
| 6 | 24.7 | FD | −/+ | +/+ | −/− | +/+ | +/+ | −/− | + | + | + | + | + | + | + | − | − | + | + | − | − |
| 7 | 36.4 | FD | −/− | −/− | −/− | −/− | −/− | −/− | − | + | + | − | − | − | − | − | − | − | − | − | − |
| 8 | 38.7 | REC | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | + | + | + | + | + | + | + | − | − | + | − | − | − |
| 9 | 41.7 | REC | +/+ | −/+ | −/+ | +/+ | NA/ NA | +/+ | + | + | − | − | + | − | − | − | − | − | − | − | |
| 10 |
42.2 |
LTH, FD |
−/− |
+/+ |
−/+ |
+/+ |
+/+ |
−/− |
+ |
+ |
NA |
+ |
+ |
+ |
− |
NA |
NA |
− |
NA |
NA |
NA |
| 3 | |||||||||||||||||||||
| 11 | 13.7# | FD | −/− | −/− | −/− | −/− | −/− | −/− | NA | − | − | − | − | − | − | − | − | − | − | − | − |
| 12 | 24.8 | REC | +/+ | −/+ | +/+ | +/+ | +/+ | +/+ | + | + | + | + | + | + | + | + | − | − | + | − | − |
| 13 | 31.0 | LTH, LBC | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | + | + | + | + | + | + | + | − | − | + | − | + | + |
| 14 | 31.4 | LBC, FD | −/− | −/+ | −/+ | −/+ | −/+ | −/− | + | + | + | + | + | + | − | + | − | − | − | NA | − |
| 15 |
43.5 |
REC |
−/+ |
−/+ |
−/+ |
−/+ |
+/+ |
+/+ |
+ |
− |
− |
− |
+ |
− |
− |
− |
− |
+ |
− |
− |
− |
| 4 direct | |||||||||||||||||||||
| 16 | 30.3 | FD | NA/ NA | −/− | −/− | −/− | −/− | −/− | − | − | − | − | − | − | − | NA | NA | − | NA | NA | NA |
| 17 | 48.9 | LBC | −/− | −/− | −/− | −/− | −/− | −/− | − | + | + | + | − | − | − | − | − | − | − | − | − |
| 18 | 57.1 | NAD | −/− | −/− | −/− | −/− | −/− | −/− | + | + | + | + | + | + | + | − | − | − | − | − | NA |
| 19 |
57.1 |
NAD |
−/− |
−/− |
−/− |
−/− |
−/− |
−/− |
NA |
− |
− |
− |
− |
− |
− |
− |
− |
− |
− |
− |
− |
| 4 indirect | |||||||||||||||||||||
| 20 | 32.7 | LBC, FD | −/+ | NA/+ | −/+ | −/+ | −/− | −/− | NA | + | NA | + | − | NA | NA | NA | NA | NA | NA | NA | NA |
| 21 |
49.8 |
Bloat |
−/− |
−/+ |
−/− |
−/− |
−/− |
−/− |
− |
+ |
+ |
+ |
+ |
+ |
+ |
− |
− |
− |
− |
− |
− |
| 4 negative controls | |||||||||||||||||||||
| 22 | 34.1 | FD | −/− | −/− | −/− | −/− | −/− | −/− | − | − | − | − | − | − | − | − | − | − | − | − | − |
| 23 | 33.4 | FD | −/− | −/− | −/− | −/− | −/− | −/− | − | − | − | − | − | − | − | − | − | − | − | − | − |
*CNS, central nervous system; FD, found dead; LBC, loss of body condition; mpc, months postinoculation; LTH, lethargy; NA, tissue not available for examination; NAD, no abnormalities detected; PrPSc, scrapie prion protein; REC, recumbency.
†Sign before the slash indicated histopathology result (presence [+] or absence [–] of spongiform change); sign after the slash indicates the PrPSc result (presence [+] or absence [–] of PrPSc deposits).
‡Presence (+) or absence (–) of PrPSc deposits as determined by immunohistochemical analysis.
§Presence (+) or absence (–) of PrPSc deposits as determined by immunohistochemical analysis.
¶Survival times for group 4 reindeer are calculated from the date of mixing of the direct controls with group 1 (reindeer challenged with chronic wasting disease from mule deer).
#Intercurrent death.
1Deceased.