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Volume 22, Number 4—April 2016
Research

Evaluation of Viremia Frequencies of a Novel Human Pegivirus by Using Bioinformatic Screening and PCR

David Bonsall, William F. Gregory, Camilla L.C. Ip, Sharyne Donfield, James Iles, M. Azim Ansari, Paolo Piazza, Amy Trebes, Anthony Brown, John Frater, Oliver G. Pybus, Phillip Goulder, Paul Klenerman, Rory Bowden, Edward D. Gomperts, Eleanor Barnes, Amit Kapoor, Colin P. Sharp, and Peter SimmondsComments to Author 
Author affiliations: University of Oxford, Oxford, UK (D. Bonsall, C.L.C. Ip, J. Iles, M.A. Ansari, P. Piazza, A. Trebes, A. Brown, J. Frater, O.G. Pybus, P. Goulder, P. Klenerman, R. Bowden, E. Barnes, P. Simmonds); University of Edinburgh Easter Bush, Edinburgh, Scotland, UK (W.F. Gregory, C.P. Sharp); Rho, Inc., Chapel Hill, North Carolina, USA (S. Donfield); The Childrens' Hospital, Los Angeles, California, USA (E.D. Gomperts); The Research Institute at Nationwide Children's Hospital, Columbus, Ohio, USA (A. Kapoor)

Main Article

Table 1

Primer sequences used for PCR screening of human pegivirus groups

Orientation Position† Sequence, 5′ → 3′
HHpgV-1/group 2 primers
Sense, outer 4,488 CGTSGTSMTYTGYGACGAGTGCCA
Antisense, outer 5,021 CCRCGCCGCTGCATVCGSAAYGC
Sense, inner 4,723 CAYGYDATCTTYTGYCACTCGAAGG
Antisense, inner
 4,900
 CRAAGTTBCCDGTGTAGCCDGTGGA
HPgV/group 1 primers
Orientation Position‡
Sense, outer 3,931 GSGCNATGGGNCCNTAYATGGA
Antisense, outer 4,546 GTNACYTCVACNACCTCCTCYACCA
Sense, inner 4,092 GTGGTNATHTGYGAYGAGTGYCA
Antisense, inner 4,357 TCRCACTCMRCCTTKGARTGRCARAA

*HHpgV, human hepegivirus; HPgV, human pegivirus.
†Position of 5′ base in the AK-790 genome (GenBank accession no. KT439329).
‡Position of 5′ base in the HPgV Iowa genome (GenBank accession no. AF121950).

Main Article

Page created: March 15, 2016
Page updated: March 15, 2016
Page reviewed: March 15, 2016
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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