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Volume 22, Number 8—August 2016
Dispatch

Survival and Growth of Orientia tsutsugamushi in Conventional Hemocultures

Sabine DittrichComments to Author , Elizabeth Card, Weerawat Phuklia, Williams E. Rudgard, Joy Silousok, Phonelavanh Phoumin, Latsaniphone Bouthasavong, Sarah Azarian, Viengmon Davong, David A.B. Dance, Manivanh Vongsouvath, Rattanaphone Phetsouvanh, and Paul N. Newton
Author affiliations: Mahosot Hospital, Vientiane, Laos (S. Dittrich, E. Card, W. Phuklia, W.E. Rudgard, J. Silousok, P. Phoumin, L. Bouthasavong, S. Azarian, V. Davong, D.A.B. Dance, M. Vongsouvath, R. Phetsouvanh, P.N. Newton); Centre for Tropical Medicine and Global Health–University of Oxford, Oxford, United Kingdom (S. Dittrick, D.A.B. Dance, R. Phetsouvanh, P.N. Newton); London School of Hygiene and Tropical Medicine, London, United Kingdom (W.E. Rudgard, S. Azarian, P.N. Newton)

Main Article

Table

Results of testing HCF from 21 patients whose samples had tested positive for Orientia tsutsugamushi by qPCR on EDTA buffy coat after >1 days of incubation, Laos, 2014*

Patient code
 Day of incubation of HCF†
1
 2
 3
 5
 7
29931 + (Cq ≈34)
30009 –
30029 –
30100 – (Cq ≈42) – + (Cq ≈35) + (Cq ≈38)
30104 + (Cq ≈38)
30316 + (Cq ≈37) – + (Cq ≈38)
30329 + (Cq≈39) + (Cq ≈35) + (Cq ≈37)
30379 + (Cq ≈34)
30416 + (Cq ≈35) + (Cq≈40) + (Cq ≈40)
30442 – –
30446 + (Cq ≈37) –
30581 – + (Cq≈32) + (Cq ≈30) + (Cq ≈34)
30600 – + (Cq ≈32)
30862 + (Cq ≈33)
30874 + (Cq ≈30)
30920
31120 + (Cq ≈31)
31199 + (Cq ≈30)
31209 + (Cq ≈31) + (Cq ≈21)
31230 + (Cq ≈31) + (Cq ≈31) + (Cq ≈32)
31231 + (Cq ≈32) + (Cq ≈30) + (Cq ≈37)

*For each patient, 2 hemocultures bottles were used (Pharmaceutical Factory No. 2, Vientiane, Laos): 1.7 g tryptic hydrolysate casein, 0.3 g soy peptone, 0.5 g sodium chloride, 0.25 g potassium phosphate, 0.25 g dextrose, 0.025 g sodium polyanetholsulfonate/100 mL) were inoculated with blood in a 1:10 dilution. Cq, cycle quantitation; HCF, hemoculture fluids; qPCR, quantitative PCR; –, sample available/negative; +, sample available/positive; blank cell, no sample available.
†Cq values are noted in parentheses to illustrate the increase in template.

Main Article

Page created: July 15, 2016
Page updated: July 15, 2016
Page reviewed: July 15, 2016
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