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Volume 22, Number 8—August 2016

Research

Phylogeographic Evidence for 2 Genetically Distinct Zoonotic Plasmodium knowlesi Parasites, Malaysia

Ruhani Yusof1, Md Atique Ahmed1, Jenarun Jelip, Hie Ung Ngian, Sahlawati Mustakim, Hani Mat Hussin, Mun Yik Fong, Rohela Mahmud, Frankie Anak Thomas Sitam, J. Rovie-Ryan Japning, Georges Snounou, Ananias A. Escalante, and Yee Ling LauComments to Author 
Author affiliations: University of Malaya Faculty of Medicine, Kuala Lumpur, Malaysia (R. Yusof, M.A. Ahmed, M.Y. Fong, R. Mahmud, Y.L. Lau); Sabah State Health Department, Sabah, Malaysia (J. Jelip); Hospital Kapit, Sarawak, Malaysia (H.U. Ngian); Hospital Tengku Microbiology Laboratory, Ampuan Rahimah, Selangor, Malaysia (S. Mustakim); Kelantan State Health Department, Kelantan, Malaysia (H.M. Hussin); Department of Wildlife and National Parks Peninsular Malaysia, Kuala Lumpur, Malaysia (F.A.T. Sitam, J. Rovie-Ryan); Sorbonne Universités, INSERM, Paris, France (G. Snounou); Temple University, Philadelphia, PA, USA (G. Snounou, A.A. Escalante)

Main Article

Table 1

Samples and sequences obtained for the PkA-type 18S rRNA gene used to distinguish 2 distinct Plasmodium knowlesi parasite populations in Malaysia*

Population location
No. sequences
Total sequences obtained
No. samples†
 Human
Macaque
Human
 Macaque
 This study‡
 GenBank§
 This study‡
 GenBank§
Peninsular Malaysia
Kelantan 13 ND
27 ND
ND ND
27
Johor 3 ND 7 ND ND ND 7
Selangor 7 5 11 ND 15 ND 26
Terengganu 2 ND 3 ND ND ND 3
Pahang 14 1 21 1 3 ND 25
Negeri Sembilan
 4
 2
 10
6
 1
 17
Malaysian Borneo
Sarawak 22 ND
48 8
ND 18 74
Sabah
 13
 ND
 30
 ND
 ND
30
Total samples 86 78 8 8 6 86 human + 14 macaque
Total sequences NA 157 9 24 19 166 human + 43 macaque

*NA, not applicable; ND, no data; PkA-type 18S rRNA, P. knowlesi type A small subunit ribosomal 18S RNA.
†Previously collected samples used for this study.
‡GenBank accession nos. KT852845–KT852938 and KJ917815–KJ917903.
§GenBank accession numbers listed in online Technical Appendix Table (http://wwwnc.cdc.gov/EID/article/22/8/15-1885-Techapp1.pdf).

Main Article

1These authors contributed equally to this article.

Page created: July 15, 2016
Page updated: July 15, 2016
Page reviewed: July 15, 2016
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