Volume 22, Number 8—August 2016
Research
Phylogeographic Evidence for 2 Genetically Distinct Zoonotic Plasmodium knowlesi Parasites, Malaysia
Table 1
Samples and sequences obtained for the PkA-type 18S rRNA gene used to distinguish 2 distinct Plasmodium knowlesi parasite populations in Malaysia*
Population location |
No. sequences |
Total sequences obtained |
||||||||
---|---|---|---|---|---|---|---|---|---|---|
No. samples† |
Human |
Macaque |
||||||||
Human |
Macaque |
This study‡ |
GenBank§ |
This study‡ |
GenBank§ |
|||||
Peninsular Malaysia | ||||||||||
Kelantan | 13 | ND | 27 | ND | ND | ND | 27 | |||
Johor | 3 | ND | 7 | ND | ND | ND | 7 | |||
Selangor | 7 | 5 | 11 | ND | 15 | ND | 26 | |||
Terengganu | 2 | ND | 3 | ND | ND | ND | 3 | |||
Pahang | 14 | 1 | 21 | 1 | 3 | ND | 25 | |||
Negeri Sembilan |
4 |
2 |
10 |
6 |
1 |
17 |
||||
Malaysian Borneo | ||||||||||
Sarawak | 22 | ND | 48 | 8 | ND | 18 | 74 | |||
Sabah |
13 |
ND |
30 |
ND |
ND |
30 |
||||
Total samples | 86 | 78 | 8 | 8 | 6 | 86 human + 14 macaque | ||||
Total sequences | NA | 157 | 9 | 24 | 19 | 166 human + 43 macaque |
*NA, not applicable; ND, no data; PkA-type 18S rRNA, P. knowlesi type A small subunit ribosomal 18S RNA.
†Previously collected samples used for this study.
‡GenBank accession nos. KT852845–KT852938 and KJ917815–KJ917903.
§GenBank accession numbers listed in online Technical Appendix Table (http://wwwnc.cdc.gov/EID/article/22/8/15-1885-Techapp1.pdf).
1These authors contributed equally to this article.