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Volume 22, Number 8—August 2016
Research

Enterovirus D68 Infection in Children with Acute Flaccid Myelitis, Colorado, USA, 2014

Negar AliabadiComments to Author , Kevin Messacar, Daniel M. Pastula, Christine C. Robinson, Eyal Leshem, James Sejvar, W. Allan Nix, M. Steven Oberste, Daniel R. Feikin, and Samuel R. Dominguez
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (N. Aliabadi, E. Leshem, J.J. Sejvar, W.A. Nix, M.S. Oberste, D.R. Feikin); Children’s Hospital Colorado, Aurora, Colorado, USA (K. Messacar, C.C. Robinson, S.R. Dominguez); Centers for Disease Control and Prevention, Fort Collins, Colorado, USA (D.M. Pastula); University of Colorado Denver, Aurora (D.M. Pastula)

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Figure 3

Sequencing results for 203 specimens from patients in a pediatric intensive care unit, Colorado, USA, 2014. All respiratory pathogen panel–positive samples were sent to the Centers for Disease Control and Prevention for further testing. Of these, 148 were positive by EV RT-PCR and 55 were negative by pan-EV RT-PCR. The 148 specimens positive by pan-EV RT-PCR were tested by EV-D68 real-time RT-PCR, and of these, 100 were positive (EV-D68). The remaining non–EV-D68 specimens were sent for molecula

Figure 3. Sequencing results for 203 specimens from patients in a pediatric intensive care unit, Colorado, USA, 2014. All respiratory pathogen panel–positive samples were sent to the Centers for Disease Control and Prevention for further testing. Of these, 148 were positive by EV RT-PCR and 55 were negative by pan-EV RT-PCR. The 148 specimens positive by pan-EV RT-PCR were tested by EV-D68 real-time RT-PCR, and of these, 100 were positive (EV-D68). The remaining non–EV-D68 specimens were sent for molecular sequencing and were identified as 26 various HRVs, 4 echoviruses, and 3 CVs; 16 specimens were not typeable. One specimen was co-infected with HRV and CV. CV, coxsackievirus; EV, enterovirus: HRV, human rhinoviruses; RT-PCR, reverse transcription PCR.

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Page created: July 15, 2016
Page updated: July 15, 2016
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