Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 23, Number 1—January 2017

Host-Associated Absence of Human Puumala Virus Infections in Northern and Eastern Germany

Stephan Drewes, Hanan Sheikh Ali, Moritz Saxenhofer, Ulrike M. Rosenfeld, Florian Binder, Fabian Cuypers, Mathias Schlegel1, Susanne Röhrs2, Gerald Heckel, and Rainer G. UlrichComments to Author 
Author affiliations: Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany (S. Drewes, H. Sheikh Ali, U.M. Rosenfeld, F. Binder, F. Cuypers, M. Schlegel, S. Röhrs, R.G. Ulrich); Sudan University of Science and Technology, Khartoum, Sudan (H. Sheikh Ali); University of Bern, Bern, Switzerland (M. Saxenhofer, G. Heckel); Swiss Institute of Bioinformatics, Lausanne, Switzerland (M. Saxenhofer, G. Heckel)

Cite This Article


Human hantavirus disease cases, caused by Puumala virus (PUUV), are mainly recorded in western and southern areas of Germany. This bank vole reservoir survey confirmed PUUV presence in these regions but its absence in northern and eastern regions. PUUV occurrence is associated with the presence of the Western bank vole phylogroup.

Puumala virus (PUUV) causes most hantavirus disease cases in Central and Western Europe and is the only human pathogenic hantavirus in Fennoscandia (1). The human infection is characterized by a mild-to-moderate form of hemorrhagic fever with renal syndrome designated nephropathia epidemica (NE), with a case fatality rate of <0.1%. The only virus reservoir in Central and Western Europe is the bank vole, Myodes glareolus (1).

Figure 1

Thumbnail of Geographic distribution of Puumala virus (PUUV)–positive and PUUV-negative bank voles in Germany (A) and assignment of bank voles to the evolutionary lineages Western, Eastern, and Carpathian (B). The coloration of the map in panel A was generated on the basis of the human PUUV incidence per district (2). PUUV detection in previous studies was extracted from (3–7). The identification of the bank vole evolutionary lineages shown in panel B was determined by using partial cytochrome b

Figure 1. Geographic distribution of Puumala virus (PUUV)–positive and PUUV-negative bank voles in Germany (A) and assignment of bank voles to the evolutionary lineages Western, Eastern, and Carpathian (B). The coloration of the...

PUUV causes most human hantavirus infections in Germany, with an incidence of 10.31 cases/100,000 inhabitants (2). Human disease reports fluctuate temporally with peaks in the years 2007, 2010, and 2012, but reports also show a heterogeneous spatial distribution (2,3). Generally and during outbreak years, the highest numbers of cases occurred in the western and southern parts of Germany, whereas in the northern and eastern parts of the country only a few cases were recorded (Figure 1, panel A).

Molecular analyses of bank voles from endemic regions detected the presence of PUUV at 30 sites in Germany (Figure 1, panel A) and resulted in the definition of several PUUV sublineages of the Central European (CE) clade (3,8). In addition, an 8-year monitoring study on the bank vole populations in a PUUV-endemic region of northwestern Germany indicated the long-term presence of particular PUUV strains (4).

To evaluate potential reasons for the almost total absence of human PUUV infections in northern and eastern Germany, we investigated bank voles from these regions and from PUUV-endemic regions in the western and southern parts of Germany for the presence of PUUV and typed the voles to major evolutionary lineages on the basis of cytochrome b gene sequences.

The Study

A total of 1,774 bank voles were collected by partners of the network Rodent-borne Pathogens (3,5,6,911) at sites in PUUV-endemic regions of western and southern Germany and sites in the eastern and northern parts of Germany (Figure 1, panel A; Technical Appendix Table). Chest cavity lavage samples of voles were investigated by IgG ELISA using a recombinant nucleocapsid protein of PUUV (6). For molecular PUUV detection, RNA was isolated from lung or heart tissue by using a QIAzol Lysis Reagent (QIAGEN, Hilden, Germany) extraction protocol. The RNA samples were subjected to small (S) segment reverse transcription PCR (RT-PCR) with primer pair Pu342F and Pu1102R (6), and the resulting cDNAs were sequenced. RNA samples were also subjected to a novel PUUV S segment–specific real-time RT-PCR with primers PUUV S-broad-F (5′-AACCCGCCATGAACAACAAC-3′) and PUUV S-broad-R (5′-TGCTGACACTGTTTGTTGCC-3′) and fluorescence reporter probe PUUV S-broad (5′ 6-FAM-GGAAATGGACCCAGATGACGT-BHQ-1 3′) (for further details see footnote of Technical Appendix Table).

First, serologic investigation of 1,758 chest cavity lavage samples indicated 99 seropositive voles exclusively originating from the endemic regions in southern and western Germany (Figure 1, panel A; Technical Appendix Table). This analysis failed to detect any antibody-positive animals within the 1,210 bank voles of this panel originating from the eastern and northern parts of Germany.

Subsequent conventional PUUV RT-PCR analysis of RNA samples from 440 voles (comprising 86 seropositive and 334 seronegative voles, 9 with equivocal results, and 11 not investigated because of the lack of chest cavity lavage samples) revealed 79 positive and 361 negative samples (Technical Appendix Table). All RT-PCR–positive samples again only originated from the PUUV-endemic regions. A final real-time RT-PCR investigation of 364 RNA samples, 34 being positive and 329 being negative by conventional RT-PCR, confirmed the results of the conventional RT-PCR analysis.

Including results of previously published studies (3,4,7), PUUV seroprevalence in the endemic regions showed an average of 23.9% and varied between 4.6% and 66.7% (Technical Appendix Table). According to the serologic and RT-PCR data, a PUUV-endemic region can be identified spanning the western and southern parts of Germany (Figure 1, panel A, below the dotted red line). In this study, the easternmost PUUV-positive sites were located in Saxony-Anhalt (site 97), Lower Saxony (site 60), and Thuringia (site 100) (7). The northernmost sites were located in Lower Saxony (sites 57 and 60) and Saxony-Anhalt (site 97). Nucleotide sequence determination and subsequent phylogenetic analysis showed that all PUUV sequences belong to the CE PUUV clade, which is divergent from other European PUUV lineages (Technical Appendix Figure).

To test for a potential association between PUUV distribution in the reservoir and evolutionary bank vole lineages, we isolated mitochondrial DNA from 383 selected voles by using the GeneMATRIX Tissue DNA Purification Kit (Roboklon, Potsdam, Germany) according to manufacturer’s guidelines. The cytochrome b PCR was performed and used for determination of the bank vole evolutionary lineages as described previously (12).

Figure 2

Thumbnail of Phylogenetic relationships of European bank vole lineages. Sequences are categorized on the basis of mitochondrial cytochrome b gene sequences and shown as a maximum clade credibility phylogenetic tree with posterior probabilities displayed for major nodes. Novel sequences are labeled with individual code and trapping site (Technical Appendix Table). Additional published sequences are included as references for bank vole evolutionary lineages, labeled with GenBank accession number f

Figure 2. Phylogenetic relationships of European bank vole lineages. Sequences are categorized on the basis of mitochondrial cytochrome b gene sequences and shown as a maximum clade credibility phylogenetic tree with posterior probabilities...

The cytochrome b–based typing revealed the presence of the bank vole Western, Eastern, and Carpathian evolutionary lineages (Figure 2). Most of the territory of Germany was inhabited by the Western evolutionary lineage, with its northern and eastern borders located close to the Elbe River (Figure 1, panel B). The distribution of the Eastern lineage ranged over almost the entire northern part of Germany, with partial sympatric occurrence of the Carpathian lineage in the northeast (sites 34, 68, 77) and the Western lineage in the central and northwest (sites 41, 52, 53, 57, 93, 97, 98). The Carpathian lineage was additionally located in the southeastern part of Germany (sites 28–30).

A comparison of the distribution of PUUV and the bank vole evolutionary lineages indicates an association of PUUV with the Western evolutionary lineage (Figure 1; Technical Appendix Table). This finding is in line with the detection of PUUV in Belgium and France and the exclusive occurrence of the Western evolutionary lineage in the PUUV-endemic regions of these countries (8,13,14). In the Bavarian Forest, the district Osnabrück (site 57), and at the easternmost distribution range in Walbeck (site 97), PUUV infections were also detected in sympatric bank voles of the Carpathian (n = 6) and Eastern (n = 7; n = 1) lineages, respectively.


The occurrence of PUUV in Germany (and Belgium and France) is preferentially associated with the presence of the Western evolutionary lineage of the bank vole, but the virus was also detected in sympatric animals of the Eastern or Carpathian lineage. Future studies will have to determine if the current distribution of PUUV can be explained by the postglacial colonization of Germany by bank voles of the Western evolutionary lineage from western refugia through southern Germany (1315).

The observed limited geographic distribution of PUUV in bank voles has important implications for public health measures and development of early warning modules for hantavirus outbreaks. These public health measures of monitoring local bank voles for PUUV strains (4) should be expanded to evaluate for further northeastern expansion.

Dr. Drewes is currently a postdoctoral researcher at the Friedrich-Loeffler-Institut in Greifswald-Insel Riems, Germany. His research interests include epidemiology and molecular evolution of hantaviruses in association with their corresponding hosts.



We kindly acknowledge Konrad Wanka, Dörte Kaufmann, Anke Mandelkow, and Marie Luisa Schmidt for their excellent assistance, our numerous collaborators within the network Rodent-borne Pathogens and Arbeitskreis Mäuse im Forst for providing bank vole samples, our colleagues involved in dissection and sample analyses, Patrick Wysocki and Nicole Neumann for creating Figure 1, and Daniela Reil for the provision of unpublished data. Collection of samples in Baden-Wuerttemberg, North Rhine-Westphalia, Thuringia, and Mecklenburg-Western Pomerania (permits 35-9185.82/0261, 8.87-, 22-2684-04-15-107/09, 7221.3-030/09) were done in the frame of UFOPLAN project 370941401.

The investigations were funded in part by the Deutsche Forschungsgemeinschaft (SPP 1596, UL 405/1-1) to R.G.U. and by grant 31003A-149585 from the Swiss National Science Foundation to G.H.



  1. Heyman  P, Ceianu  CS, Christova  I, Tordo  N, Beersma  M, João Alves  M, et al. A five-year perspective on the situation of haemorrhagic fever with renal syndrome and status of the hantavirus reservoirs in Europe, 2005-2010. Euro Surveill. 2011;16:97786.PubMedGoogle Scholar
  2. Robert Koch-Institut. SurvStat@RKI 2.0. 2016 [cited 2016 Jan12].
  3. Ettinger  J, Hofmann  J, Enders  M, Tewald  F, Oehme  RM, Rosenfeld  UM, et al. Multiple synchronous outbreaks of Puumala virus, Germany, 2010. Emerg Infect Dis. 2012;18:14614.DOIPubMedGoogle Scholar
  4. Weber de Melo  V, Sheikh Ali  H, Freise  J, Kühnert  D, Essbauer  S, Mertens  M, et al. Spatiotemporal dynamics of Puumala hantavirus associated with its rodent host, Myodes glareolus. Evol Appl. 2015;8:54559.DOIPubMedGoogle Scholar
  5. Essbauer  SS, Schmidt-Chanasit  J, Madeja  EL, Wegener  W, Friedrich  R, Petraityte  R, et al. Nephropathia epidemica in metropolitan area, Germany. Emerg Infect Dis. 2007;13:12713.DOIPubMedGoogle Scholar
  6. Mertens  M, Kindler  E, Emmerich  P, Esser  J, Wagner-Wiening  C, Wölfel  R, et al. Phylogenetic analysis of Puumala virus subtype Bavaria, characterization and diagnostic use of its recombinant nucleocapsid protein. Virus Genes. 2011;43:17791.DOIPubMedGoogle Scholar
  7. Faber  M, Wollny  T, Schlegel  M, Wanka  KM, Thiel  J, Frank  C, et al. Puumala virus outbreak in Western Thuringia, Germany, 2010: epidemiology and strain identification. Zoonoses Public Health. 2013;60:54954.DOIPubMedGoogle Scholar
  8. Castel  G, Couteaudier  M, Sauvage  F, Pons  JB, Murri  S, Plyusnina  A, et al. Complete genome and phylogeny of Puumala hantavirus isolates circulating in France. Viruses. 2015;7:547688.DOIPubMedGoogle Scholar
  9. Schilling  S, Emmerich  P, Klempa  B, Auste  B, Schnaith  E, Schmitz  H, et al. Hantavirus disease outbreak in Germany: limitations of routine serological diagnostics and clustering of virus sequences of human and rodent origin. J Clin Microbiol. 2007;45:300814.DOIPubMedGoogle Scholar
  10. Mertens  M, Essbauer  SS, Rang  A, Schröder  J, Splettstoesser  WD, Kretzschmar  C, et al. Non-human primates in outdoor enclosures: risk for infection with rodent-borne hantaviruses. Vet Microbiol. 2011;147:4205.DOIPubMedGoogle Scholar
  11. Morger  J, Råberg  L, Hille  SM, Helsen  S, Štefka  J, Al-Sabi  MM, et al. Distinct haplotype structure at the innate immune receptor toll-like receptor 2 across bank vole populations and lineages in Europe. Biol J Linn Soc Lond. 2015;116:12433. DOIGoogle Scholar
  12. Ali  HS, Drewes  S, Sadowska  ET, Mikowska  M, Groschup  MH, Heckel  G, et al. First molecular evidence for Puumala hantavirus in Poland. Viruses. 2014;6:34053.DOIPubMedGoogle Scholar
  13. Wójcik  JM, Kawałko  A, Marková  S, Searle  JB, Kotlík  P. Phylogeographic signatures of northward post-glacial colonization from high-latitude refugia: a case study of bank voles using museum specimens. J Zool (Lond). 2010;281:24962.
  14. Filipi  K, Marková  S, Searle  JB, Kotlík  P. Mitogenomic phylogenetics of the bank vole Clethrionomys glareolus, a model system for studying end-glacial colonization of Europe. Mol Phylogenet Evol. 2015;82(Pt A):24557.DOIPubMedGoogle Scholar
  15. Kotlík  P, Deffontaine  V, Mascheretti  S, Zima  J, Michaux  JR, Searle  JB. A northern glacial refugium for bank voles (Clethrionomys glareolus). Proc Natl Acad Sci U S A. 2006;103:148604.DOIPubMedGoogle Scholar




Cite This Article

DOI: 10.3201/eid2301.160224

1Current affiliation: Seramun Diagnostica GmbH, Heidesee, Germany.

2Current affiliation: Stiftung Tierärztliche Hochschule Hannover, Hannover, Germany.

Table of Contents – Volume 23, Number 1—January 2017

EID Search Options
presentation_01 Advanced Article Search – Search articles by author and/or keyword.
presentation_01 Articles by Country Search – Search articles by the topic country.
presentation_01 Article Type Search – Search articles by article type and issue.



Please use the form below to submit correspondence to the authors or contact them at the following address:

Rainer G. Ulrich, Friedrich-Loeffler-Institut, Institute for Novel and Emerging Infectious Diseases, Südufer 10, 17493 Greifswald-Insel Riems, Germany

Send To

10000 character(s) remaining.


Page created: December 14, 2016
Page updated: December 14, 2016
Page reviewed: December 14, 2016
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.