Volume 23, Number 10—October 2017
Dispatch
Berlin Squirrelpox Virus, a New Poxvirus in Red Squirrels, Berlin, Germany
Gudrun Wibbelt1, Simon H. Tausch1, Piotr W. Dabrowski, Olivia Kershaw, Andreas Nitsche
, and Livia Schrick
, and Livia Schrick
Table
Results of PCRs of different tissues from 7 live and 4 deceased squirrels showing poxvirus lesions, Berlin, Germany*
| Year of sampling | Live/dead | Tissue | Cq BerSQPV | Cq c-myc | ΔCq (BerSQPV − c-myc) |
|---|---|---|---|---|---|
| 2014 |
Dead |
Archived skin (paraffin) |
23.7 |
34.8 |
−11.1 |
| 2015 |
Live |
Crust‡ |
12.5 |
17.7 |
−5.2 |
| 2015 |
Live |
Crust‡ |
14.8 |
18.3 |
−3.5 |
| 2015 |
Dead |
Skin (foot)‡ | 11.1 | 18.8 | −7.7 |
| Skin (tail) | 9.7 | 17.9 | −8.2 | ||
| Skin (toe)†‡ | 10.1 | 18.6 | −8.5 | ||
| Lung | 33.2 | 27.0 | 6.2 | ||
| Liver | 34.7 | 23.1 | 11.6 | ||
| Spleen | 34.9 | 23.9 | 11.0 | ||
| Brain |
33.9 |
24.5 |
9.4 |
||
| 2015 |
Dead |
Skin (forefoot)‡ | 10.9 | 18.2 | −7.3 |
| Skin | 26.3 | 28.0 | −1.7 | ||
| Lung | 33.6 | 23.1 | 10.5 | ||
| Liver | Negative | 22.1 | NA | ||
| Spleen | 38.3 | 23.9 | 14.4 | ||
| Kidney | Negative | 24.1 | NA | ||
| Small intestine | Negative | 21.8 | NA | ||
| Large intestine | Negative | 24.4 | NA | ||
| Brain |
Negative |
25.3 |
NA |
||
| 2015 |
Dead |
Crust | 19.0 | 23.2 | −4.2 |
| Lung | 35.2 | 25.4 | 9.8 | ||
| Liver | Negative | 20.8 | NA | ||
| Spleen | 34.0 | 25.1 | 8.9 | ||
| Kidney | Negative | 25.9 | NA | ||
| Small intestine | 36.4 | 21.6 | 14.8 | ||
| Large intestine | 35.0 | 23.5 | 11.5 | ||
| Brain |
Negative |
24.6 |
NA |
||
| 2016 |
Live |
Crust |
15.0 |
22.0 |
−7.0 |
| 2016 |
Live |
Crust |
12.1 |
18.6 |
−6.5 |
| 2016 |
Live |
Crust |
14.1 |
20.8 |
−6.7 |
| 2016 |
Live |
Crust |
13.2 |
17.7 |
−4.5 |
| 2016 | Live | Crust | 12.9 | 18.3 | −5.4 |
*BerSQPV DNA was quantified in relation to cellular c-myc DNA; lower values for ΔCq indicate higher virus loads in a respective tissue. BerSQPV, Berlin squirrelpox virus; Cq, quantification cycle; NA, not applicable.
†Specimen applied to next-generation sequencing
‡Specimen used to obtain the cell culture isolate
1These authors contributed equally to this article.
Page created: September 18, 2017
Page updated: September 18, 2017
Page reviewed: September 18, 2017
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