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Volume 23, Number 10—October 2017

Dispatch

Berlin Squirrelpox Virus, a New Poxvirus in Red Squirrels, Berlin, Germany

Gudrun Wibbelt1, Simon H. Tausch1, Piotr W. Dabrowski, Olivia Kershaw, Andreas NitscheComments to Author , and Livia Schrick
Author affiliations: Leibniz Institute for Zoo and Wildlife Research, Berlin, Germany (G. Wibbelt); Robert Koch Institute, Berlin (S.H. Tausch, P.W. Dabrowski, A. Nitsche, L. Schrick); Free University Berlin, Berlin (O. Kershaw)

Main Article

Table

Results of PCRs of different tissues from 7 live and 4 deceased squirrels showing poxvirus lesions, Berlin, Germany*

Year of sampling Live/dead Tissue Cq BerSQPV Cq c-myc ΔCq (BerSQPV − c-myc)
2014
Dead
Archived skin (paraffin)
23.7
34.8
−11.1
2015
Live
Crust‡
12.5
17.7
−5.2
2015
Live
Crust‡
14.8
18.3
−3.5
2015
Dead
Skin (foot)‡ 11.1 18.8 −7.7
Skin (tail) 9.7 17.9 −8.2
Skin (toe)†‡ 10.1 18.6 −8.5
Lung 33.2 27.0 6.2
Liver 34.7 23.1 11.6
Spleen 34.9 23.9 11.0
Brain
33.9
24.5
9.4
2015
Dead
Skin (forefoot)‡ 10.9 18.2 −7.3
Skin 26.3 28.0 −1.7
Lung 33.6 23.1 10.5
Liver Negative 22.1 NA
Spleen 38.3 23.9 14.4
Kidney Negative 24.1 NA
Small intestine Negative 21.8 NA
Large intestine Negative 24.4 NA
Brain
Negative
25.3
NA
2015
Dead
Crust 19.0 23.2 −4.2
Lung 35.2 25.4 9.8
Liver Negative 20.8 NA
Spleen 34.0 25.1 8.9
Kidney Negative 25.9 NA
Small intestine 36.4 21.6 14.8
Large intestine 35.0 23.5 11.5
Brain
Negative
24.6
NA
2016
Live
Crust
15.0
22.0
−7.0
2016
Live
Crust
12.1
18.6
−6.5
2016
Live
Crust
14.1
20.8
−6.7
2016
Live
Crust
13.2
17.7
−4.5
2016 Live Crust 12.9 18.3 −5.4

*BerSQPV DNA was quantified in relation to cellular c-myc DNA; lower values for ΔCq indicate higher virus loads in a respective tissue. BerSQPV, Berlin squirrelpox virus; Cq, quantification cycle; NA, not applicable.
†Specimen applied to next-generation sequencing
‡Specimen used to obtain the cell culture isolate

Main Article

1These authors contributed equally to this article.

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