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Volume 23, Number 12—December 2017
Synopsis

Fatal Outbreak in Tonkean Macaques Caused by Possibly Novel Orthopoxvirus, Italy, January 20151

Giusy Cardeti2Comments to Author , Cesare Ernesto Maria Gruber2, Claudia Eleni, Fabrizio Carletti, Concetta Castilletti, Giuseppe Manna, Francesca Rosone, Emanuela Giombini, Marina Selleri, Daniele Lapa, Vincenzo Puro, Antonino Di Caro, Raniero Lorenzetti, Maria Teresa Scicluna, Goffredo Grifoni, Annapaola Rizzoli, Valentina Tagliapietra, Lorenzo De Marco, Maria Rosaria Capobianchi, and Gian Luca Autorino
Author affiliations: Istituto Zooprofilattico Sperimentale del Lazio e della Toscana M. Aleandri, Rome, Italy (G. Cardeti, C. Eleni, G. Manna, F. Rosone, R. Lorenzetti, M.T. Scicluna, G. Grifoni, G.L. Autorino); L, Spallanzani National Institute of Infectious Diseases, Rome (C.E.M. Gruber, F. Carletti, C. Castilletti, E. Giombini, M. Selleri, D. Lapa, V. Puro, A. Di Caro, M.R. Capobianchi); Fondazione Edmund Mach di San Michele all’Adige, Trento, Italy (A. Rizzoli, V. Tagliapietra); Parco Faunistico Piano dell'Abatino, Poggio San Lorenzo, Italy (L. De Marco)

Main Article

Figure 4

Results from necropsy of Tonkean macaque (Macaca tonkeana) from animal sanctuary, Italy, January 2015. A) Hematoxylin and eosin stain of cutaneous lesion. Focal epidermal necrosis, acanthosis ballooning degeneration, and acantholysis of keratinocytes was observed. Staining shows early vesiculation with eosinophilic intracytoplasmic inclusion bodies (arrows) in enlarged degenerated cells. B) Electron micrograph of skin lesion sample showing negatively stained brick-shaped viral particle of ≈160–2

Figure 4. Results from necropsy of Tonkean macaque (Macaca tonkeana) from animal sanctuary, Italy, January 2015. A) Hematoxylin and eosin stain of cutaneous lesion. Focal epidermal necrosis, acanthosis ballooning degeneration, and acantholysis of keratinocytes was observed. Staining shows early vesiculation with eosinophilic intracytoplasmic inclusion bodies (arrows) in enlarged degenerated cells. B) Electron micrograph of skin lesion sample showing negatively stained brick-shaped viral particle of ≈160–220 nm, consistent with orthopoxvirus. Scale bar = 100 nm. C) SYBR Green (ThermoFisher Scientific, Waltham, MA, USA) real-time PCR melting curve of all tested samples. The y-axis shows the ratio of the change in fluorescence over the change in temperature. The average melting temperature (80.8°C ± 1°C) was consistent with that for the orthopoxvirus genome.

Main Article

1Preliminary results from this study were presented at the Xth International Congress of the European Society for Veterinary Virology; August 31–September 3, 2015; Montpellier, France.

2These first authors were co–principal investigators who contributed equally to this article.

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Page updated: November 16, 2017
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