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Volume 23, Number 12—December 2017
Dispatch

Pathogenic Elizabethkingia miricola Infection in Cultured Black-Spotted Frogs, China, 2016

Ruixue Hu, Junfa Yuan, Yin Meng, Zhe Wang, and Zemao GuComments to Author 
Author affiliations: Huazhong Agricultural University, Wuhan, China (R. Hu, J. Yuan, Y. Meng, Z. Wang, Z. Gu); Hubei Engineering Research Center for Aquatic Animal Diseases Control and Prevention, Wuhan (R. Hu, J. Yuan, Z. Gu)

Main Article

Table 2

Results of the experimental exposure of frogs to Elizabethkingia miricola isolate FL160902, China, 2016*

Route of infection
 Concentration, CFU/mL
 No. frogs
per trial
 Cumulative no. deaths, by days after exposure† Mortality, %
2
 4
 6
 8
 10
 12
 14
Intramuscular injection‡ 105 10 0 1 1 1 1 1 1 10
106 10 0 0 1 1 5 5 5 50
107 10 1 3 6 7 7 7 7 70
SPSS§
 10
 0
 0
 0
 0
 0
 0
 0
 0
Immersion inoculation¶
 106
 10
 3
 7
 10
 10
 10
 10
 10
 100
Cohabitation inoculation#
 NA
 10
 0
 0
 1
 3
 3
 3
 3
 30
Control NA 10 0 0 0 0 0 0 0 0

*NA, not applicable.
†Deaths after 14 d were not included.
‡Injection volume 200 μL.
§An equivalent volume injection of 0.70% stroke-physiologic saline solution.
¶Immersed for 30 min in E. miricola suspension.
#Frogs in this trial cohabited with frogs previously infected with E. miricola.

Main Article

Page created: November 16, 2017
Page updated: November 16, 2017
Page reviewed: November 16, 2017
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