Volume 23, Number 12—December 2017
Dispatch
Phylogenetic Characterization of Crimean-Congo Hemorrhagic Fever Virus, Spain
Table
Primer pair |
5′ → 3′ sequence |
5′ → 3′ position relative to reference strain AB1-2009 |
Amplified segment, kb |
Temperature, °C† |
CRCON+1 | RWAAYGGRCTTRTGGAYACYTTCAC | 123–147 | S, 1.4 | 50.5 |
CRCON1R-S |
TCTCAAAGATAYCGTTGCCGCAC |
1,651–1,673 |
||
CRCON1F-M | TCTCAAAGAAAATACTTGCGGC | 1–22 | M, 2.3 | 48 |
CRCON4R-M | GCATYTCAGCATCTATTGCATT | 2,326–22,347 | ||
CRCONF4F-M | TTGTAGAYCAAAGACTRGGCAG | 1,775–1,796 | M, 2.1 | 48 |
CRCON6R-M |
GCCYGCTTCAATCAAGCTACA |
3,829-3,849 |
||
CRCON6F-M | TCAATTGAGGCACCATGGGG | 3,280–3,299 | M, 2.1 | 50 |
CRCON8R-M |
TCTCAAAGATATAGTGGCGGCA |
5,348–5,369 |
||
CRCON1F-L | TCTCAAAGATATCAATCCCCCC | 1–22 | L, 2.2 | 48 |
CRCON3R-L |
AGTGTCGAAAATGTRCAAATCTC |
2,202--2224 |
||
CRCON3F-L | CCTGAAAGTGACCTCACCCGC | 1,562–1,582 | L, 3.1 | 55 |
CRCON6R-L |
TTGGCATGCTTGCAGGGCTTAG |
4,656–4,677 |
||
CRCON7F-L | TGCTTGCAGGGCTTAGTAGGCT | 4,661–4,682 | L, 2.3 | 50.5 |
CRCON9R-L |
TCATGCATGCAACCACTGAAAT |
6,948-6,969 |
||
CCHF-L2F | GAAGAGCTATATGACATAAGGC | 6,137–6,158 | L, 1.6 | 48 |
CCHF-L1R |
TTGGCACTATCTTTCATTTGAC |
7,752–7,773 |
||
CRCON10F-L | GGTAGTTCAGATGATTACGCAAA | 7,616–7,638 | L, 1.4 | 48 |
CRCON10R-L |
CCTGTTAATTGTTTGCCACAA |
9,019–9,039 |
||
CRCON7200F | ATGCAACAGGTTCTGAAAAATG | 7,199–7,220 | L, 3.5 | 48 |
CRCON11R-L |
ATGCTCCTAGTGATGCCATAATG |
10,743–10,765 |
||
CRCON12F-L | TCTTTTGAAGGTGAAGCATCTTG | 10,585–10,607 | L, 1.6 | 48 |
CCHF-L2R |
TCTCAAAGAAATCGTTCCCCCCAC |
12,149–12,172 |
||
*F, forward; L, large segment; M, medium segment; R, reverse; RT-PCR, reverse transcription PCR; S, small segment. †Melting temperature used. |
1These authors contributed equally to this article.
Page created: November 16, 2017
Page updated: November 16, 2017
Page reviewed: November 16, 2017
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.