Figure 3. Amino acid substitutions in respiratory syncytial virus A (RSV-A) G protein for sequences isolated in Kilifi Kenya from season 2011/2012 to 2014/2015. All unique protein sequences per epidemic were collated, aligned and the amino acid differences from the earliest sequence determined and marked with vertical colored bars, with the substituted amino acid residue color coded as shown by the key between panels A and B. A) Full aligned aa sequence inferred from the G gene sequences (ON1 and GA2); B) (ON1 only) focuses on the region of the ON1 duplication. The positions shown at the bottom of panels A and B are relative to the first amino acid of the regions analyzed, i.e., from amino acid positions 94 and 260, respectively, of the reference strain A2 (Ref: M74568). Indicated at the top of these panels are the functional domains of the G protein (panel A) and the 72-nt duplication of genotype ON1 (panel B; duplicated sequence I in “orange” and duplicated sequence II in “purple”). Below this, the histogram indicates the total number of changes at each position. C) Concurrent aa positions within the duplicated sequences I and II, and the respective aa substitutions (numbering similar to positions in panel B).