Volume 23, Number 5—May 2017
Research
Lack of Durable Cross-Neutralizing Antibodies Against Zika Virus from Dengue Virus Infection
Table 1
DENV and Zika virus neutralization profiles for persons with travel history to Zika virus–endemic areas*
Serostatus, serum sample ID |
Place of Infection |
IC50† |
||||
DENV-1 |
DENV-2 |
DENV-3 |
DENV-4 |
Zika virus |
||
Primary DENV-1 | ||||||
147 | Latin America | 3,552 | 287 | 557 | 75 | <20 |
153 | Latin America | 757 | <20 | <20 | <20 | <20 |
05/262 | Asia | 274 | <20 | <20 | <20 | <20 |
06/125 | Asia | 3,823 | 222 | 125 | 80 | <20 |
99/1230 |
Asia |
1,219 |
63 |
30 |
24 |
<20 |
Primary DENV-2 | ||||||
001 | Asia | 49 | 2,188 | 48 | 89 | <20 |
08/90 | Asia | <20 | 2,966 | <20 | <20 | <20 |
08/91 | Asia | <20 | 838 | <20 | <20 | <20 |
09/165 | Asia | <20 | 2,093 | <20 | <20 | <20 |
09/251 |
Asia |
<20 |
417 |
<20 |
<20 |
<20 |
Primary DENV-3 | ||||||
116 | Asia | 200 | 979 | 5,342 | 290 | <20 |
118 | Latin America | 173 | 374 | 3,041 | 56 | <20 |
125 | Latin America | 99 | 97 | 1,648 | 35 | <20 |
133 | Latin America | 89 | 171 | 3,348 | 83 | <20 |
06/297 |
Asia |
27 |
<20 |
573 |
<20 |
<20 |
Primary DENV-4 | ||||||
112 | Latin America | 908 | 1367 | 591 | 18,408 | <20 |
06/105 | Asia | <20 | <20 | <20 | 941 | <20 |
06/302 | Asia | <20 | <20 | <20 | 4,130 | <20 |
09/159 |
Asia |
115 |
226 |
478 |
5,694 |
<20 |
Primary Zika virus | ||||||
168 | Latin America | 36ǂ | <20 | 78‡ | <20 | 1,382 |
172 |
Latin America |
<20 |
<20 |
<20 |
<20 |
8,468 |
Secondary DENV | ||||||
000 | Asia | 3,306 | 2,087 | 1,162 | 782 | <20 |
003 | Asia | 556 | 178 | 299 | <20 | 146 |
115 | Asia | 100 | 355 | 830 | 245 | <20 |
141 | Latin America | 1,902 | 1,953 | 4,530 | 664 | <20 |
144 | Asia | 155 | 191 | 5,782 | 1,612 | 699 |
145 | Asia | 601 | 1,262 | 240 | 60 | <20 |
146 | Asia | 403 | 1,052 | 1,480 | 451 | 28 |
155 | Asia | 215 | 299 | 71 | 27 | <20 |
160 | Latin America | 947 | 3,564 | 131 | 1,600 | <20 |
06/123 | Asia | 1,776 | 827 | 82 | 157 | <20 |
06/124 | Asia | 1,454 | 1,208 | 1,673 | 1,011 | <20 |
09/157 | Asia | 282 | 1,104 | 73 | 134 | <20 |
09/250 |
Asia |
375 |
1475 |
151 |
94 |
<20 |
Secondary Zika virus | ||||||
165 | Latin America | 60‡ | 79‡ | 70‡ | 508 | 1655 |
166 | Latin America | 929 | 393 | 4344 | 240 | 1814 |
*DENV, dengue virus; IC50, 50% inhibitory concentration; ID, identification.
†All neutralization testing performed on Vero cells with the exception of DT003, which was limiting and historic values performed on a U937 Flow-based assay are shown for DENV-1–4. Zika virus IC50 values for DT003 are from Vero assays.
‡Curves that did not pass quality metrics are marked. Such curves might signify very low titer IC50 values or variable background at the lower limit of detection in serum with no specific neutralization activity.