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Volume 23, Number 6—June 2017
Dispatch

Isolated Case of Marburg Virus Disease, Kampala, Uganda, 2014

Luke Nyakarahuka, Joseph Ojwang, Alex Tumusiime, Stephen Balinandi, Shannon Whitmer, Simon Kyazze, Sam Kasozi, Milton Wetaka, Issa Makumbi, Melissa Dahlke, Jeff Borchert, Julius Lutwama, Ute Ströher, Pierre E. Rollin, Stuart T. Nichol, and Trevor R. ShoemakerComments to Author 
Author affiliations: Uganda Virus Research Institute, Entebbe, Uganda (L. Nyakarahuka, J. Lutwama); US Centers for Disease Control and Prevention, Entebbe (J. Ojwang, A. Tumusiime, S. Balinandi, J. Borchert, T.R. Shoemaker); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (S. Whitmer, U. Ströher, P.E. Rollin, S.T. Nichol); Public Health Emergency Operations Center, Kampala, Uganda (S. Kyazze, S. Kasozi, M. Wetaka, I. Makumbi, M. Dahlke)

Main Article

Table

Primers used to generate Marburg virus-specific cDNA fragments for whole-genome sequencing of isolates from Uganda

Genome fragment
 Primer set
 Primer sequence, 5’→3’
 Region amplified
 Region size, kbp
1 63F TGA CAT TGA GAC TTG TCA GTC 64-4998 4.9
MARB-4997R
 GCT TGA TTT CCT TCA CGC
2 3005F AAG TCA GCG AGG GGT TGA TGA CTG GAA AAG 2970-6438 3.5
6426R
 TGC TAT GTT CCC TTC AGT GAA GAC
3 6101F AGA AAA CAG AAG ACG TCC ATC TGA TG 6065-9405 3.3
9405R
 ACT TAA TGC TGC ACG AAG TGA TG
4 7567F TGG CCC TGG AAT IGA AGG ACT C 7548-10571 3.0
10571R
 AGC ATA TGA ACA ATA GAT C
5 36F GTA CCT CTA AGG AAA ATC ATG AAG 9979-15442 5.4
57R
 GTT GAT ATA ATT GCA CGT GTA GAT
6 12006F TTG CCA GAA GGA TAA AAG GAC AAA GAG 11953-15486 3.5
15516R
 ATT TTG GAA GAT TAT ATT ACT ATC
7 15519F TGG ACG ATA GGA AAT CGA GCA C 15108-19111 4.0
19155R TGG ACA CAC TAA AAA GAT G

Main Article

Page created: May 16, 2017
Page updated: May 16, 2017
Page reviewed: May 16, 2017
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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