Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

Volume 23, Number 7—July 2017

Dispatch

Postmortem Findings for 7 Neonates with Congenital Zika Virus Infection

Figures

Tables

Technical Appendices

Altmetric

Anastácio Q. SousaComments to Author , Diane I.M. Cavalcante, Luciano M. Franco, Fernanda M.C. Araújo, Emília T. Sousa, José Telmo Valença-Junior, Dionne B. Rolim, Maria E.L. Melo, Pedro D.T. Sindeaux, Marialva T.F. Araújo, Richard D. Pearson, Mary E. Wilson, and Margarida M.L. Pompeu
Author affiliations: Federal University of Ceará, Fortaleza, Brazil (A.Q. Sousa, D.I.M. Cavalcante, L.M. Franco, E.T. Sousa, J.T. Valença-Junior, P.D.T. Sindeaux, M.M.L. Pompeu); Serviço de Verificação de Óbitos-SVO, Fortaleza (L.M. Franco, E.T. Sousa, J.T. Valença-Junior); Ceará State Central Public Health Laboratory, Fortaleza (F.M.C. Araújo, M.E.L. Melo); University of Fortaleza, Fortaleza (D.B. Rolim); Ceará State Secretariat of Health, Fortaleza (D.B. Rolim); Evandro Chagas Institute, Belém, Brazil (M.T.F. Araújo); University of Virginia School of Medicine, Charlottesville, Virginia, USA (R.D. Pearson); University of California, San Francisco, California, USA (M.E. Wilson); Harvard T.H. Chan School of Public Health, Boston, Massachusetts, USA (M.E. Wilson)

Cite This Article

Close

Highlight and copy the desired format.

EID Sousa AQ, Cavalcante D, Franco LM, Araújo F, Sousa ET, Valença-Junior J, et al. Postmortem Findings for 7 Neonates with Congenital Zika Virus Infection. Emerg Infect Dis. 2017;23(7):1164-1167. https://dx.doi.org/10.3201/eid2307.162019
AMA Sousa AQ, Cavalcante D, Franco LM, et al. Postmortem Findings for 7 Neonates with Congenital Zika Virus Infection. Emerging Infectious Diseases. 2017;23(7):1164-1167. doi:10.3201/eid2307.162019.
APA Sousa, A. Q., Cavalcante, D., Franco, L. M., Araújo, F., Sousa, E. T., Valença-Junior, J....Pompeu, M. (2017). Postmortem Findings for 7 Neonates with Congenital Zika Virus Infection. Emerging Infectious Diseases, 23(7), 1164-1167. https://dx.doi.org/10.3201/eid2307.162019.

Abstract

Postmortem examination of 7 neonates with congenital Zika virus infection in Brazil revealed microcephaly, ventriculomegaly, dystrophic calcifications, and severe cortical neuronal depletion in all and arthrogryposis in 6. Other findings were leptomeningeal and brain parenchymal inflammation and pulmonary hypoplasia and lymphocytic infiltration in liver and lungs. Findings confirmed virus neurotropism and multiple organ infection.

From the discovery of Zika virus in Uganda in 1947 through 2007, when an outbreak occurred on Yap Island, Micronesia, only sporadic cases of human infection had been reported (1). In early 2015, the virus emerged in Brazil (2). Its role in a major public health crisis became apparent when links between Zika virus infection and microcephaly and Guillain-Barré syndrome were established (1). Cases of microcephaly associated with Zika virus have been well documented (37). We report a case series of postmortem findings that strengthen this association.

The Study

Postmortem examinations were performed on 7 neonates from the state of Ceará, in northeastern Brazil. Their mothers most likely contracted Zika virus infection during the first trimester of pregnancy in early 2015. Six of the families lived in small towns far from the capital of Fortaleza, suggesting that Zika virus was widespread in Ceará at that time.

During November 2015–February 2016, the 7 autopsies were performed by the Service for Ascertaining Death (Ceará, Brazil). Real-time reverse transcription PCR (RT-PCR) of cerebrospinal fluid and tissue, performed at the Central Public Health Laboratory (Ceará), confirmed congenital Zika virus infection (8) (Table). We reviewed medical charts from live patients and their mothers; autopsy reports; and histopathologic reports from 4 pathologists who reviewed the hematoxylin and eosin–stained slides of brain, cerebellum, lung, heart, liver, spleen, kidney, and bladder. Samples were also tested for dengue virus by real-time RT-PCR and for dengue virus nonstructural protein 1 and IgM (9,10) (Table). After consent was obtained from families, the autopsies were performed as routine cause-of-death investigations.

Of the 7 mothers, all were HIV negative and 5 had had symptoms compatible with viral infection during the first trimester of pregnancy. Neonate survival times ranged from 30 minutes to 6 days after birth; 5 survived <1 hour, 1 died 48 hours after birth, and 1 survived for 6 days. Four neonates were male; for 1 of these in whom the genitalia were ambiguous (neonate 2), the sex was ascertained by identification of undescended testes at autopsy. Gestational ages ranged from 30 to 42 weeks (median 37 weeks) (Technical Appendix [PDF - 28 KB - 1 page]).

Figure 1

Thumbnail of Physical signs in 4 of 7 neonates who died of congenital Zika virus infection, Brazil. A) Neonate 1: typical microcephaly phenotype; arthrogryposis in upper and lower limbs. B) Neonate 7: microcephaly without the typical microcephaly phenotype; arthrogryposis is also present. C) Neonate 3: typical microcephaly phenotype, with head circumference within reference limits, frontal view. D) Neonate 3: typical microcephaly phenotype, with head circumference within reference limits, profil

Figure 1. Physical signs in 4 of 7 neonates who died of congenital Zika virus infection, Brazil. A) Neonate 1: typical microcephaly phenotype; arthrogryposis in upper and lower limbs. B) Neonate 7: microcephaly...

Brain weight was decreased in all neonates; however, body weight was within reference range for gestational age in all except neonate 6 (online Technical Appendix). For 6 neonates, microcephaly was present (Figure 1, panels A, B). One neonate for whom head circumference was within reference limits had morphologic changes typical of microcephaly (Figure 1, panels C, D). Cerebellar hypoplasia was present in neonates 1 and 2 (Figure 1, panel E) and pachygyria in neonate 3 (Figure 1, panel F). Ventriculomegaly was present in all neonates (Figure 1, panels G, H) and arthrogryposis in 6 (Figure 1, panels A, B).

Figure 2

Thumbnail of Histologic slides of tissues from 4 of 7 neonates who died of congenital Zika virus infection, Brazil. A) Neonate 1: severe cortical thinning (3 mm) with subventricular dystrophic calcification, reactive gliosis, and marked leptomeningeal congestion as well as marked depletion of neuronal precursors (original magnification ×10). B) Neonate 1: severe thinning of brain parenchyma (0.8 mm) with striking depletion of neuronal precursors (original magnification ×10). C) Neonate 1: lympho

Figure 2. Histologic slides of tissues from 4 of 7 neonates who died of congenital Zika virus infection, Brazil. A) Neonate 1: severe cortical thinning (3 mm) with subventricular dystrophic calcification, reactive gliosis,...

All infants had thinning of the brain parenchyma with severe depletion of neuronal precursors (Figure 2, panel A). In some areas of the brain, the distance from the meningeal membranes to the ependymal epithelium was 3.0 mm (Figure 2, panel A); in 1 neonate, it measured only 0.8 mm (Figure 2, panel B).

Lymphocytic inflammation was observed in the meninges of 5 neonates (Figure 2, panels B, C) and in the brain parenchyma of 5; for 3 neonates, inflammatory processes were found in both areas. Areas of inflammation varied in intensity and distribution, but periventricular and perivascular cuffing were common (Figure 2, panel D). There was moderate to severe vascular congestion in 6 neonates (Figure 1, panels E, F; Figure 2, panel E). Macroscopic calcification was common (Figure 1, panel G). Three patterns of dystrophic calcification were identified microscopically: individual neuronal mineralization, a fine granular pattern (Figure 2, panel F), and a more coarse dystrophic pattern (Figure 2, panels A, E, G). Coarse calcification in a band-like form (Figure 2, panels A, G) was seen mainly at the junction of gray and white matter and in periventricular areas in association with the inflammatory process. Dystrophic calcification with neuronal mineralization was seen in all neonates. A variable degree of gliosis was present in all neonates, affecting predominantly white matter. Red neurons, neurons with increased cytoplasmic eosinophilia (reflecting acute neuronal injury), were seen in 6 neonates (Figure 2, panel H), and apoptosis was seen in 5 (online Technical Appendix). Additional findings included foci of brain hemorrhage, mainly in periventricular areas.

Pulmonary hypoplasia was present in all neonates (online Technical Appendix); relative lung weight (lung weight/body weight) was 0.004–0.01 g (11). Intra-alveolar hemorrhage was seen in 3 neonates; bleeding was severe in neonates 5 and 7. Interstitial lymphocytic pulmonary infiltration (Figure 2, panel I) and expansion of alveolar septa (Figure 2, panel J) was present in neonate 1, who died soon after birth. Fresh frozen lung tissue from that neonate was positive for Zika virus by RT-PCR (Table).

Liver specimens were available from 6 neonates; moderate to severe hydropic degeneration was found in 5 of these specimens. Round eosinophilic cytoplasmic structures suggestive of megamitochondria were observed in 2 neonates, mild to moderate steatosis and Councilman bodies (apoptosis) in 3, hepatocyte necrosis in 1 (neonate 7) (online Technical Appendix), and mild periportal lymphocytic inflammation in 1 (neonate 2).

Kidneys were available from 6 of the neonates. Focal glomerular sclerosis was present in neonates 2 and 6. In neonate 2, tissue was positive for Zika virus by RT-PCR (Table). Moderate lymphocytic cystitis was present in tissue from 1 available bladder. No histologic abnormalities were found on any of the hearts or spleens. Placental tissues were available from 4 neonates; common findings were fibrinoid necrosis, chorangiosis, and amnion hyperplasia. Dengue virus 1 was detected by RT-PCR in cerebrospinal fluid from neonate 4 (Table).

Conclusions

Of the 7 Zika virus–infected neonates examined, 6 did not show intrauterine growth restriction, but all 7 had remarkably decreased brain weight (online Technical Appendix), emphasizing the neurotropism of Zika virus. These findings are similar to those earlier reported for congenital Zika virus infection (37). In contrast, an animal model of congenital Zika virus infection with similar neuropathologic damage was associated with striking global growth retardation (12).

The constellation of neuropathologic features (ventriculomegaly, mineralized neurons, and dystrophic calcification with band-like subcortical distribution) differs from features seen in other common infections associated with congenital abnormalities (e.g., TORCH [toxoplasmosis, other viruses, rubella, cytomegalovirus and herpesvirus infections]) and should raise suspicion for congenital Zika virus infection, warranting further workup. For instance, the pattern of calcifications seen in tissue from patients with congenital cytomegalovirus infection and in toxoplasmosis are predominantly periventricular (13).

Pulmonary hypoplasia, defined as lung weight:body weight ratio of <0.012 (1.2%), seemed to be a major factor determining death during the perinatal period (11). The occurrence of pulmonary hypoplasia and arthrogryposis was most likely a part of fetal akinesia deformation sequence, resulting primarily from central nervous system damage (14).

In addition, variable liver damage, a finding commonly seen with infection by other flaviviruses, was found in these neonates (15). The detection of Zika virus in tissues that did not show pathologic abnormalities could be the result of viremia or of the tissues sampled; the pathologic abnormalities were mostly focal (samples for PCR could have differed from those used for histopathologic examination). Another possibility could be cross-contamination during the sample collection.

Our report confirms not only the neurotropism of the virus but also the occurrence of pathologic changes consistent with viral infection in multiple organs: liver (Councilman bodies and periportal lymphocytic infiltration), lungs (interstitial lymphocytic pneumonitis), and bladder (lymphocytic cystitis). PCR detection of Zika virus in liver, lung, and kidney tissue also strengthened our hypothesis that Zika virus can infect multiple tissues.

Dr. Sousa is an infectious disease specialist and an associate professor of clinical medicine and pathology at the Federal University of Ceará, Brazil. His primary research interests are leishmaniasis and other emerging infectious diseases of northeastern Brazil.

Acknowledgment

We thank Pedro S.C. Ciarlini for his contribution to the draft of the article and the neuropathologic analysis.

References

  1. Musso D, Gubler DJ. Zika Virus. Clin Microbiol Rev. 2016;29:487524. DOIPubMed
  2. Zanluca C, Melo VC, Mosimann AL, Santos GI, Santos CN, Luz K. First report of autochthonous transmission of Zika virus in Brazil. Mem Inst Oswaldo Cruz. 2015;110:56972. DOIPubMed
  3. Mlakar J, Korva M, Tul N, Popović M, Poljšak-Prijatelj M, Mraz J, et al. Zika virus associated with microcephaly. N Engl J Med. 2016;374:9518. DOIPubMed
  4. Driggers RW, Ho C-Y, Korhonen EM, Kuivanen S, Jääskeläinen AJ, Smura T, et al. Zika virus infection with prolonged maternal viremia and fetal brain abnormalities. N Engl J Med. 2016;374:214251. DOIPubMed
  5. Sarno M, Sacramento GA, Khouri R, do Rosário MS, Costa F, Archanjo G, et al. Zika virus infection and stillbirths: a case of hydrops fetalis, hydranencephaly and fetal demise. PLoS Negl Trop Dis. 2016;10:e0004517. DOIPubMed
  6. Perez S, Tato R, Cabrera JJ, Lopez A, Robles O, Paz E, et al. Confirmed case of Zika virus congenital infection, Spain, March 2016. Euro Surveill. 2016;21:30261. DOIPubMed
  7. Martines RB, Bhatnagar J, de Oliveira Ramos AM, Davi HP, Iglezias SD, Kanamura CT, et al. Pathology of congenital Zika syndrome in Brazil: a case series. Lancet. 2016;388:898904. DOIPubMed
  8. Lanciotti RS, Kosoy OL, Laven JJ, Velez JO, Lambert AJ, Johnson AJ, et al. Genetic and serologic properties of Zika virus associated with an epidemic, Yap State, Micronesia, 2007. Emerg Infect Dis. 2008;14:12329. DOIPubMed
  9. Lima MR, Nogueira RMR, Schatzmayr HG, dos Santos FB. Comparison of three commercially available dengue NS1 antigen capture assays for acute diagnosis of dengue in Brazil. PLoS Negl Trop Dis. 2010;4:e738. DOIPubMed
  10. Araújo FMC, Brilhante RSN, Cavalcanti LPG, Rocha MFG, Cordeiro RA, Perdigão ACB, et al. Detection of the dengue non-structural 1 antigen in cerebral spinal fluid samples using a commercially available enzyme-linked immunosorbent assay. J Virol Methods. 2011;177:12831. DOIPubMed
  11. Wigglesworth JS, Desai R. Use of DNA estimation for growth assessment in normal and hypoplastic fetal lungs. Arch Dis Child. 1981;56:6015. DOIPubMed
  12. Cugola FR, Fernandes IR, Russo FB, Freitas BC, Dias JL, Guimarães KP, et al. The Brazilian Zika virus strain causes birth defects in experimental models. Nature. 2016;534:26771.PubMed
  13. Folkerth RD, Kinney HC. Disorders of the perinatal period. In: Love S, Louis DN, Ellison DW, editors. Greenfield's Neuropathology. 8th ed., vol. 1. Boca Raton (FL): CRC Press; 2008. p. 241–315.
  14. Kowalczyk B, Feluś J. Arthrogryposis: an update on clinical aspects, etiology, and treatment strategies. Arch Med Sci. 2016;12:1024. DOIPubMed
  15. Aye KS, Charngkaew K, Win N, Wai KZ, Moe K, Punyadee N, et al. Pathologic highlights of dengue hemorrhagic fever in 13 autopsy cases from Myanmar. Hum Pathol. 2014;45:122133. DOIPubMed

Figures

Table

Technical Appendix

Cite This Article

DOI: 10.3201/eid2307.162019

Table of Contents – Volume 23, Number 7—July 2017

Comments

Please use the form below to submit correspondence to the authors or contact them at the following address:

Anastácio Q. Sousa, Department of Clinical Medicine, School of Medicine, Federal University of Ceará. Rua Prof. Costa Mendes, 1608 - 4° andar - Rodolfo Teófilo, CEP 60.430-140, Fortaleza, Ceará, Brazil


character(s) remaining.

Comment submitted successfully, thank you for your feedback.

TOP