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Volume 24, Number 5—May 2018
Dispatch

External Quality Assessment for Zika Virus Molecular Diagnostic Testing, Brazil

Carlo Fischer, Celia Pedroso, Alfredo Mendrone, Ana Maria Bispo de Filippis, Antonio Carlos Rosário Vallinoto, Bergmann Morais Ribeiro, Edison Luiz Durigon, Ernesto T.A. Marques, Gubio S. Campos, Isabelle F.T. Viana, José Eduardo Levi, Luciano Cesar Scarpelli, Mauricio Lacerda Nogueira, Michele de Souza Bastos, Nathalia C. Santiago Souza, Ricardo Khouri, Sanny M. Costa Lira, Shirley Vasconcelos Komninakis, Cécile Baronti, Rémi N. Charrel, Beate M. Kümmerer, Christian Drosten, Carlos Brites, Xavier de Lamballerie, Matthias Niedrig, Eduardo Martins Netto, and Jan Felix DrexlerComments to Author 
Author affiliations: German Centre for Infection Research, associated partner Charité–Universitätsmedizin, Berlin, Germany (C. Fischer, C. Drosten, J.F. Drexler); LAPI, Hospital Universitário Professor Edgard Santos, Salvador, Brazil (C. Pedroso, C. Brites, E.M. Netto); Fundação Pro-Sangue/Hemocentro de São Paulo, São Paulo, Brazil (A. Mendrone Jr., J.E. Levi); Instituto Oswaldo Cruz, Rio de Janeiro, Brazil (A.M.B. de Filippis); Federal University of Para, Belém, Brazil (A.C.R. Vallinoto); University of Brasília, Brasília, Brazil (B. Morais Ribeiro); University of São Paulo, São Paulo (E.L. Durigon, J.E. Levi, N.C.S. Souza); Oswaldo Cruz Foundation, Pernambuco, Brazil (E.T.A. Marques Jr., I.F.T. Viana); Universidade Federal da Bahia, Salvador (G.S. Campos); Diagnósticos da América—DASA, São Paulo (J.E. Levi, L.C. Scarpelli); Hospital Israelita Albert Einstein, São Paulo (J.E. Levi, S.M.C. Lira); Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, Brazil (M.L. Nogueira); Fundação de Medicina Tropical Dr. Heitor Vieira Dourado, Manaus, Brazil (M. de Souza Bastos); Fundação Oswaldo Cruz, Salvador, Brazil (R. Khouri); Federal University of São Paulo, São Paulo (S.V. Komninakis); Aix Marseille Université, Marseille, France (C. Baronti, R.N. Charrel, X. de Lamballerie); Assistance Publique-Hopitaux Marseille, Marseille (C. Baronti, R.N. Charrel, X. de Lamballerie); University of Bonn Medical Centre, Bonn, Germany (B.M. Kümmerer); Robert Koch Institute, Berlin, Germany (M. Niedrig)

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Figure 1

External quality assessment (EQA) performance and lower limits of detection (LODs) for Zika virus molecular diagnostic testing, Brazil. A) EQA performance of individual laboratories. Gray bars above the baseline indicate correctly tested samples; bars below the baseline indicate incorrectly tested samples. Laboratories are sorted by the quantity of correct samples and the numeric order of the laboratory identification numbers. Laboratory 8 tested only 9 of 12 samples. B) Projected 95% LODs of pa

Figure 1. External quality assessment (EQA) performance and lower limits of detection (LODs) for Zika virus molecular diagnostic testing, Brazil. A) EQA performance of individual laboratories. Gray bars above the baseline indicate correctly tested samples; bars below the baseline indicate incorrectly tested samples. Laboratories are sorted by the quantity of correct samples and the numeric order of the laboratory identification numbers. Laboratory 8 tested only 9 of 12 samples. B) Projected 95% LODs of participating laboratories under optimal conditions; C) projected 95% LODs of participating laboratories assuming a 5-fold loss in sensitivity. LODs were projected using the technical LOD of the Lanciotti et al. assay as analyzed previously (2), input and elution volumes, and real-time reverse transcription-PCR setups. Efficacy of RNA extraction was assumed to be 100%. Whiskers indicate 95% CIs. Dotted line indicates the lowest Zika virus RNA titer of an EQA specimen. Laboratories are grouped according to their EQA performance as excellent, medium, or problematic. LODs did not differ significantly among groups (p>0.05 by Kruskal-Wallis test)..

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Page created: April 17, 2018
Page updated: April 17, 2018
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