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Volume 24, Number 5—May 2018
Dispatch

External Quality Assessment for Zika Virus Molecular Diagnostic Testing, Brazil

Carlo Fischer, Celia Pedroso, Alfredo Mendrone, Ana Maria Bispo de Filippis, Antonio Carlos Rosário Vallinoto, Bergmann Morais Ribeiro, Edison Luiz Durigon, Ernesto T.A. Marques, Gubio S. Campos, Isabelle F.T. Viana, José Eduardo Levi, Luciano Cesar Scarpelli, Mauricio Lacerda Nogueira, Michele de Souza Bastos, Nathalia C. Santiago Souza, Ricardo Khouri, Sanny M. Costa Lira, Shirley Vasconcelos Komninakis, Cécile Baronti, Rémi N. Charrel, Beate M. Kümmerer, Christian Drosten, Carlos Brites, Xavier de Lamballerie, Matthias Niedrig, Eduardo Martins Netto, and Jan Felix DrexlerComments to Author 
Author affiliations: German Centre for Infection Research, associated partner Charité–Universitätsmedizin, Berlin, Germany (C. Fischer, C. Drosten, J.F. Drexler); LAPI, Hospital Universitário Professor Edgard Santos, Salvador, Brazil (C. Pedroso, C. Brites, E.M. Netto); Fundação Pro-Sangue/Hemocentro de São Paulo, São Paulo, Brazil (A. Mendrone Jr., J.E. Levi); Instituto Oswaldo Cruz, Rio de Janeiro, Brazil (A.M.B. de Filippis); Federal University of Para, Belém, Brazil (A.C.R. Vallinoto); University of Brasília, Brasília, Brazil (B. Morais Ribeiro); University of São Paulo, São Paulo (E.L. Durigon, J.E. Levi, N.C.S. Souza); Oswaldo Cruz Foundation, Pernambuco, Brazil (E.T.A. Marques Jr., I.F.T. Viana); Universidade Federal da Bahia, Salvador (G.S. Campos); Diagnósticos da América—DASA, São Paulo (J.E. Levi, L.C. Scarpelli); Hospital Israelita Albert Einstein, São Paulo (J.E. Levi, S.M.C. Lira); Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, Brazil (M.L. Nogueira); Fundação de Medicina Tropical Dr. Heitor Vieira Dourado, Manaus, Brazil (M. de Souza Bastos); Fundação Oswaldo Cruz, Salvador, Brazil (R. Khouri); Federal University of São Paulo, São Paulo (S.V. Komninakis); Aix Marseille Université, Marseille, France (C. Baronti, R.N. Charrel, X. de Lamballerie); Assistance Publique-Hopitaux Marseille, Marseille (C. Baronti, R.N. Charrel, X. de Lamballerie); University of Bonn Medical Centre, Bonn, Germany (B.M. Kümmerer); Robert Koch Institute, Berlin, Germany (M. Niedrig)

Main Article

Table 2

Viral RNA preparation of individual laboratories in an external quality assessment for Zika virus molecular diagnostic testing, Brazil

Lab ID Extraction method Extraction kit Input volume, μL Elution volume, μL PCR template volume, μL
1 Manual QIAamp Viral RNA Mini Kit (QIAGEN, São Paulo, Brazil) 140 50 10
2 Manual QIAamp Viral RNA Mini Kit (QIAGEN) 200 200 5
3 Automated QIAsymphony DSP Virus/Pathogen Midi Kit (QIAGEN) 200† 60 8.8
4 Automated Maxwell 16 Viral Total Nucleic Acid Purification Kit (Promega, São Paulo, Brazil) 140 50 5
5 Manual QIAamp Viral RNA Mini Kit (QIAGEN) 140 60 5
6 Automated QIAsymphony DSP Virus/Pathogen Kit (QIAGEN) 200 100 5
7 Manual QIAamp Viral RNA Mini Kit (QIAGEN) 140 60 4.5
8 Automated Maxwell 16 Viral Total Nucleic Acid Purification Kit (Promega) 150 50 5
9 Manual High Pure Viral Nucleic Acid Kit (Roche, São Paulo, Brazil) 200 50 1
10 Manual QIAamp Viral RNA Mini Kit (QIAGEN) 160 50 5
11 Automated NucliSENS easyMAG Kit (bioMérieux, Rio de Janeiro, Brazil) 200† 50 10
12 Automated Magna Pure Compact Nucleic Acid Isolation Kit I—Large Volume (Roche) 200‡ 50 5
13 Automated Maxwell 16 Viral Total Nucleic Acid Purification Kit (Promega) 100 50 5
14 Manual QIAamp Viral RNA Mini Kit (QIAGEN) 140 60 5
15 Automated Abbot mSample Preparation System RNA (4 × 24 prep) (Abbott, São Paulo, Brazil) 200 80 5

*All details are listed as declared by the participants. ID, identification.
†Laboratory that filled the 200 µL provided in this external quality assessment panel to higher standard extraction input volumes ranging from 500 µL to 1,200 µL using human plasma tested negative for arboviral infections beforehand.
‡Laboratory that filled the 200 µL provided in this external quality assessment panel to higher standard extraction input volumes ranging from 500 µL to 1,200 µL using sterile nuclease-free water.

Main Article

Page created: April 17, 2018
Page updated: April 17, 2018
Page reviewed: April 17, 2018
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