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Volume 25, Number 11—November 2019
Dispatch

Non-Leishmania Parasite in Fatal Visceral Leishmaniasis–Like Disease, Brazil

Sandra R. Maruyama1Comments to Author , Alynne K.M. de Santana12, Nayore T. Takamiya, Talita Y. Takahashi, Luana A. Rogerio, Caio A.B. Oliveira, Cristiane M. Milanezi, Viviane A. Trombela, Angela K. Cruz, Amélia R. Jesus, Aline S. Barreto, Angela M. da Silva, Roque P. Almeida3, José M. Ribeiro3, and João S. Silva3
Author affiliations: Universidade Federal de São Carlos, São Carlos, Brazil (S.R. Maruyama, N.T. Takamiya, T.Y. Takahashi, L.A. Rogerio, C.A.B. Oliveira); Universidade Federal de Sergipe, Aracaju, Brazil (A.K.M. de Santana, A.R. Jesus, A.S. Barreto, A.M. da Silva, R.P. Almeida); Universidade de São Paulo, Ribeirão Preto, Brazil (C.M. Milanezi, V.A. Trombela, A.K. Cruz); National Institutes of Health, Rockville, Maryland, USA (J.M. Ribeiro); Fundação Oswaldo Cruz Bi-institucional, Ribeirão Preto (J.S. Silva)

Main Article

Figure 2

Experimental infection of BALB/c mice with LVH60 and LVH60a clinical isolates obtained from a 64-year-old man with fatal visceral leishmaniasis–like illness, Brazil. LVH60 was isolated from bone marrow, LVH60a from a skin lesion biopsy. Female BALB/c mice were infected intravenously with 107 stationary-phase promastigotes. After 4 weeks of infection, spleen and liver samples were collected. Parasite loads were determined by a limiting dilution assay of spleen and liver homogenates and are expres

Figure 2. Experimental infection of BALB/c mice with LVH60 and LVH60a clinical isolates obtained from a 64-year-old man with fatal visceral leishmaniasis–like illness, Brazil. LVH60 was isolated from bone marrow, LVH60a from a skin lesion biopsy. Female BALB/c mice were infected intravenously with 107 stationary-phase promastigotes. After 4 weeks of infection, spleen and liver samples were collected. Parasite loads were determined by a limiting dilution assay of spleen and liver homogenates and are expressed as the mean ± SD. A) LVH60 strain infection in mice resulted in parasite detection in the spleen and liver; the LVH60a strain was not detected in the spleen. B) For cutaneous infection, BALB/c mice were injected subcutaneously in the right ear dermis with 106 stationary phase promastigotes. Infected ears were collected and imaged. C) Parasite burden in ears was assessed by a limiting dilution assay. D) Ear thickness was measured weekly with a digital caliper. The HU-UFS14 strain (L. infantum) was used as a positive control for experimental visceral leishmaniasis (A), whereas the LV29 strain (L. major) was used as a positive control for experimental cutaneous leishmaniasis. The results represent 3 independent experiments. Error bars indicate SD. ND, not detected. *p<0.05.

Main Article

1These authors contributed equally to this article.

2Current affiliation: Universidade de São Paulo, Ribeirão Preto, Brazil.

3These senior authors contributed equally to this article.

Page created: October 15, 2019
Page updated: October 15, 2019
Page reviewed: October 15, 2019
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