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Volume 25, Number 11—November 2019
Dispatch

Isolation of Legionella pneumophila by Co-culture with Local Ameba, Canada

Rafik DeyComments to Author , Harley Mount, Alex W. Ensminger, Greg J. Tyrrell, Linda P. Ward, and Nicholas J. Ashbolt
Author affiliations: University of Alberta, Edmonton, Alberta, Canada (R. Dey, G.J. Tyrrell, N.J. Ashbolt); University of Toronto, Toronto, Ontario, Canada (H. Mount, A.W. Ensminger); Alberta Health Services, Edmonton (G.J. Tyrrell, L.P. Ward, N.J. Ashbolt)

Main Article

Figure 2

Phylogenetic tree depicting the relationship between Legionella pneumophila isolates identified during investigation of legionellosis in an immunocompromised 3-year-old girl, Calgary, Alberta, Canada, and reference sequences. L. pneumophila core ortholog-based maximum-likelihood phylogenetic tree shows 8 previously published genomes and sequences of the 2 isolates from this study (2017a, clinical isolate from patient; 2017b, environmental isolate from hot tub in patient’s home). Tree constructio

Figure 2. Phylogenetic tree depicting the relationship between Legionella pneumophila isolates identified during investigation of legionellosis in an immunocompromised 3-year-old girl, Calgary, Alberta, Canada, and reference sequences. L. pneumophila core ortholog-based maximum-likelihood phylogenetic tree shows 8 previously published genomes and sequences of the 2 isolates from this study (2017a, clinical isolate from patient; 2017b, environmental isolate from hot tub in patient’s home). Tree construction was performed by using 2,403 orthologous sequences (2,471,034 nt). Each ortholog sequence was independently aligned with the MUSCLE algorithm (https://www.ebi.ac.uk/Tools/msa/muscle) and concatenated into a single superalignment, which then was subjected to 1,000 bootstrap iterations to find the maximum-likelihood phylogeny. Scale bar indicates the number of nucleotide substitutions per site. ST, sequence type.

Main Article

Page created: October 15, 2019
Page updated: October 15, 2019
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