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Volume 25, Number 11—November 2019
Dispatch

Isolation of Legionella pneumophila by Co-culture with Local Ameba, Canada

Rafik DeyComments to Author , Harley Mount, Alex W. Ensminger, Greg J. Tyrrell, Linda P. Ward, and Nicholas J. Ashbolt
Author affiliations: University of Alberta, Edmonton, Alberta, Canada (R. Dey, G.J. Tyrrell, N.J. Ashbolt); University of Toronto, Toronto, Ontario, Canada (H. Mount, A.W. Ensminger); Alberta Health Services, Edmonton (G.J. Tyrrell, L.P. Ward, N.J. Ashbolt)

Main Article

Figure 1

Transmission electron micrograph at of amebae isolated from the home hot tub of a an immunocompromised 3-year-old girl with legionellosis before and after coculture with Legionella pneumophila, Calgary, Alberta, Canada. A) Trophozoites of Vermamoeba vermiformis before coculture. Note the absence of intracellular bacteria in the replicative phagosome. B) V. vermiformis replicative phagosome containing L. pneumophila serogroup 6 after 6 h of coculture. Arrows indicate L. pneumophila contained with

Figure 1. Transmission electron micrograph of amebae isolated from the home hot tub of a an immunocompromised 3-year-old girl with legionellosis before and after co-culture with Legionella pneumophila, Calgary, Alberta, Canada. A) Trophozoites of Vermamoeba vermiformis before co-culture. Note the absence of intracellular bacteria in the replicative phagosome. B) V. vermiformis replicative phagosome containing L. pneumophila serogroup 6 after 6 h of co-culture. Arrows indicate L. pneumophila contained within replicative phagosomes. Scale bars in left panels indicate 2 μm; scale bars in right panels indicate 500 nm. cv, contractile vacuoles; m, mitochondria; N, nucleus; rp, replicative phagosome.

Main Article

Page created: October 15, 2019
Page updated: October 15, 2019
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