Volume 25, Number 2—February 2019
Cytauxzoon felis Infection in Domestic Cats, Yunnan Province, China, 2016
We performed a molecular survey for Cytauxzoon felis infection in 311 domestic cats in Yunnan Province, China, in 2016 and found a prevalence of 21.5%. C. felis infection in domestic and wild cats in other provinces should be investigated to determine parasite prevalence and genetic diversity among cats throughout China.
Cytauxzoonosis is a tickborne hemoprotozoal disease of both domestic cats and wild felids caused mainly by Cytauxzoon felis protozoa (1,2). In the late 1900s, C. felis protozoa were reported exclusively in North America, particularly in the mid-Atlantic states of the United States (3), but in the early 2000s, this pathogen was reported in some countries of South America, and in Europe, several other Cytauxzoon species were identified (4). Cytauxzoonosis of domestic cats has long been considered contagious and deadly (2). However, as research progressed, the virulence of different C. felis isolates was found to be inconsistent; some cats were able to survive the infection and potentially serve as natural reservoirs (5).
The number of pet cats around the world is increasing, but the information about the prevalence of C. felis infection in domestic cats is limited worldwide. Because of the seriousness of feline cytauxzoonosis and its geographic expansion to more and more regions, informing veterinarians, pet owners, and the general public about this disease has become imperative. The objective of this study was to examine whether C. felis infection is present in domestic cats in China.
During November–December 2016, we collected whole blood from the femoral vein of 311 domestic cats (74 stray cats and 237 pet cats) in Yunnan Province in southwestern China using EDTA tubes. We stored these EDTA whole blood samples at −20°C and then performed genomic DNA extraction with the TIANamp Genomic DNA Kit (TianGen, http://www.tiangen.com) following the manufacturer’s protocol. To detect C. felis infection, we performed a PCR targeting the second internal transcribed spacer (ITS-2) of ribosomal DNA (6). We sequenced amplicons in both directions and compared these sequences with those of other relevant C. felis isolates available in GenBank. We analyzed differences in C. felis prevalence in domestic cats according to lifestyle, region, sex, and age using the χ2 test in SPSS 22.0 standard version for Windows (IBM Corporation, https://www.ibm.com). We considered differences statistically significant when the p value obtained was <0.05.
In total, 67 (21.5%) of 311 examined domestic cats were positive for the C. felis protozoan. We sequenced these C. felis–positive PCR products and obtained 67 ITS-2 sequences; 4 representative sequences were deposited in GenBank (accession nos. MF966369–72). The 67 C. felis ITS-2 sequences shared 98.4%–100% similarity. These sequences had 95.6%–100% similarity with corresponding C. felis ITS-2 sequences available in GenBank.
The prevalence of C. felis protozoa in domestic cats in Yunnan Province was 21.5% (Table), lower than the prevalence in domestic cats in the United States (30.3%, 27/89) (7) but higher than that in Brazil (0.66%, 1/151) (8). The C. felis prevalence in stray cats (51.4%, 38/74) was significantly higher (p<0.001) than that in pet cats (12.2%, 29/237) (Table), probably because stray cats often live outdoors with poor sanitation, thus having high probability of contact with ticks. However, no significant difference in C. felis prevalence was found among domestic cats of different sexes or age groups.
Distinct C. felis genotypes of different virulences in domestic cats have been identified, and genetic diversity among C. felis populations has been studied by comparisons of 18S rRNA, ITS-1, and ITS-2 sequences (1). ITS-1 and ITS-2 rDNA are better genetic markers for assessing C. felis genotypic variability (9) because these sequences evolve faster than the 18S rRNA gene. A combination of ITS-1 and ITS-2 sequences has been used to identify the C. felis genotypes present in various domestic cats and wild felids (1).
C. felis protozoa are transmitted to domestic cats by ticks, such as Amblyomma americanum and Dermacentor variabilis (1). Raising pet cats indoors and preventing and treating ectoparasites of outdoor stray cats would help reduce risk for infection in C. felis protozoa–endemic areas. Some effective antitick insecticides can be used for preventing transmission of this parasite (10).
Our study revealed a high (21.5%) C. felis prevalence in domestic cats in Yunnan Province, China. Further studies are warranted to assess the prevalence of the C. felis protozoan in wild felids and domestic cats in other regions of China to estimate its geographic distribution and genetic diversity and to investigate its potential tick vectors.
Dr. Zou is professor of veterinary parasitology at the Key Laboratory of Veterinary Public Health of Higher Education of Yunnan Province, College of Veterinary Medicine, Yunnan Agricultural University, Kunming, Yunnan, China. His research focuses on the epidemiology and molecular biology of parasitic protozoa and control strategies for parasitic infections of animals and humans.
This study was supported by the Excellent Scientist Fund of Yunnan Agricultural University (grant no. 2015JY03), the Open Funds of the State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (grant no. SKLVEB2017KFKT008), the Elite Program of Chinese Academy of Agricultural Sciences, and the Agricultural Science and Technology Innovation Program (grant no. CAAS-ASTIP-2016-LVRI-03).
- Wang JL, Li TT, Liu GH, Zhu XQ, Yao C. Two tales of Cytauxzoon felis infections in domestic cats. Clin Microbiol Rev. 2017;30:861–85. DOIPubMedGoogle Scholar
- Sherrill MK, Cohn LA. Cytauxzoonosis: diagnosis and treatment of an emerging disease. J Feline Med Surg. 2015;17:940–8. DOIPubMedGoogle Scholar
- Birkenheuer AJ, Le JA, Valenzisi AM, Tucker MD, Levy MG, Breitschwerdt EB. Cytauxzoon felis infection in cats in the mid-Atlantic states: 34 cases (1998–2004). J Am Vet Med Assoc. 2006;228:568–71. DOIPubMedGoogle Scholar
- Carli E, Trotta M, Chinelli R, Drigo M, Sinigoi L, Tosolini P, et al. Cytauxzoon sp. infection in the first endemic focus described in domestic cats in Europe. Vet Parasitol. 2012;183:343–52. DOIPubMedGoogle Scholar
- Rizzi TE, Reichard MV, Cohn LA, Birkenheuer AJ, Taylor JD, Meinkoth JH. Prevalence of Cytauxzoon felis infection in healthy cats from enzootic areas in Arkansas, Missouri, and Oklahoma. Parasit Vectors. 2015;8:13. DOIPubMedGoogle Scholar
- Brown HM, Latimer KS, Erikson LE, Cashwell ME, Britt JO, Peterson DS. Detection of persistent Cytauxzoon felis infection by polymerase chain reaction in three asymptomatic domestic cats. J Vet Diagn Invest. 2008;20:485–8. DOIPubMedGoogle Scholar
- Brown HM, Lockhart JM, Latimer KS, Peterson DS. Identification and genetic characterization of Cytauxzoon felis in asymptomatic domestic cats and bobcats. Vet Parasitol. 2010;172:311–6. DOIPubMedGoogle Scholar
- André MR, Herrera HM, de Jesus Fernandes S, de Sousa KCM, Gonçalves LR, Domingos IH, et al. Tick-borne agents in domesticated and stray cats from the city of Campo Grande, state of Mato Grosso do Sul, midwestern Brazil. Ticks Tick Borne Dis. 2015;6:779–86. DOIPubMedGoogle Scholar
- Shock BC, Birkenheuer AJ, Patton LL, Olfenbuttel C, Beringer J, Grove DM, et al. Variation in the ITS-1 and ITS-2 rRNA genomic regions of Cytauxzoon felis from bobcats and pumas in the eastern United States and comparison with sequences from domestic cats. Vet Parasitol. 2012;190:29–35. DOIPubMedGoogle Scholar
- Reichard MV, Thomas JE, Arther RG, Hostetler JA, Raetzel KL, Meinkoth JH, et al. Efficacy of an imidacloprid 10%/flumethrin 4.5% collar (Seresto, Bayer) for preventing the transmission of Cytauxzoon felis to domestic cats by Amblyomma americanum. Parasitol Res. 2013;112(Suppl 1):11–20. DOIPubMedGoogle Scholar
TableCite This Article
Original Publication Date: January 07, 2019
1These authors contributed equally to this article.
Table of Contents – Volume 25, Number 2—February 2019
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Please use the form below to submit correspondence to the authors or contact them at the following address:
Xing-Quan Zhu, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 1 Xujiaping, Yanchangbu, Lanzhou, Gansu 730046, China