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Volume 26, Number 11—November 2020
Research

Endotheliopathy and Platelet Dysfunction as Hallmarks of Fatal Lassa Fever

Lucy E. Horton1, Robert W. Cross1, Jessica N. Hartnett, Emily J. Engel, Saori Sakabe, Augustine Goba, Mambu Momoh, John Demby Sandi, Thomas W. Geisbert, Robert F. Garry, John S. Schieffelin, Donald S. Grant, and Brian M. SullivanComments to Author 
Author affiliations: The Scripps Research Institute, La Jolla, California, USA (L.E. Horton, S. Sakabe, B.M. Sullivan); University of Texas Medical Brach, Galveston, Texas, USA (R.W. Cross, T.W. Geisbert); Tulane University School of Medicine, New Orleans, Louisiana, USA (J.N. Hartnett, E.J. Engel, R.F. Garry, J.S. Schieffelin); Kenema Government Hospital, Kenema, Sierra Leone (A. Goba, M. Momoh, J.D. Sandi, D.S. Grant); Ministry of Health and Sanitation, Freetown, Sierra Leone (A. Goba, M. Momoh, J.D. Sandi); Eastern Polytechnic Institute, Kenema (M. Momoh, D.S. Grant); Njala University, Moyamba, Sierra Leone (J.D. Sandi); University of Sierra Leone, Freetown (D.S. Grant)

Main Article

Figure 7

Representative platelet aggregometry performed on a 1:1 mix of platelet-rich plasma from a healthy control (HC) participant and platelet-poor plasma dialyzed to remove EDTA from either healthy controls or acute Lassa fever (LF) patients, Sierra Leone, 2015–2018. Aggregation was stimulated by addition of 5 μmol ADP. Plasma from fatal LF cases caused a decrease in aggregation at 4 min compared with peak aggregation, but plasma from LF survivor and non–LF febrile controls (NLFCs) showed no disaggregation by 4 min. A) Percent aggregation over 4 min. B) Aggregation at 4 min divided by the maximum aggregation in assays by using plasma from 14 fatal LF cases, 7 nonfatal LF cases, and 5 NLFC cases. Only assays using plasma from fatal cases showed disaggregation by the experimental endpoint. Error bars shows SD; horizontal lines indicate means. HC, healthy control; LF, Lassa fever; NLFC, non-LF febrile control.

Figure 7. Representative platelet aggregometry performed on a 1:1 mix of platelet-rich plasma from a healthy control (HC) participant and platelet-poor plasma dialyzed to remove EDTA from either healthy controls or acute Lassa fever (LF) patients, Sierra Leone, 2015–2018. Aggregation was stimulated by addition of 5 μmol ADP. Plasma from fatal LF cases caused a decrease in aggregation at 4 min compared with peak aggregation, but plasma from LF survivor and non–LF febrile controls (NLFCs) showed no disaggregation by 4 min. A) Percent aggregation over 4 min. B) Aggregation at 4 min divided by the maximum aggregation in assays by using plasma from 14 fatal LF cases, 7 nonfatal LF cases, and 5 NLFC cases. Only assays using plasma from fatal cases showed disaggregation by the experimental endpoint. Error bars shows SD; horizontal lines indicate means. HC, healthy control; LF, Lassa fever; NLFC, non-LF febrile control.

Main Article

1These authors contributed equally to this article.

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