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Volume 26, Number 12—December 2020
Dispatch

Differential Tropism of SARS-CoV and SARS-CoV-2 in Bat Cells

Susanna K.P. Lau1Comments to Author , Antonio C.P. Wong1, Hayes K.H. Luk1, Kenneth S.M. Li, Joshua Fung, Zirong He, Flora K.K. Cheng, Tony T.Y. Chan, Stella Chu, Kam Leng Aw-Yong, Terrence C.K. Lau, Kitty S.C. Fung, and Patrick C.Y. WooComments to Author 
Author affiliations: The University of Hong Kong, Hong Kong, China (S.K.P. Lau, A.C.P. Wong, H.K.K. Luk, K.S.M. Li, J. Fung, Z. He, F.K.K. Cheng, T.T.Y. Chan, S. Chu, K.L. Aw-Yong, P.C.Y. Woo); City University of Hong Kong, Hong Kong (T.C.K. Lau); United Christian Hospital, Kwun Tong, Hong Kong (K.S.C. Fung)

Main Article

Table 1

Primers used for reverse transcription quantitative PCR in study of coronavirus in bats*

Target Primers, 5¢ ® 3¢
Forward Reverse Probe
SARS-CoV N gene
CDC_N3
 GGGAGCCTTGAATACACCAAAA
 TGTAGCACGATTGCAGCATTG
 (FAM)
AYCACATTGGCACCCGCAATCCTG (BHQ1)
β-actin CTCTTCCAGCCCTCCTTCCT (for bat cells) or
CTCTTCCAGCCTTCCTTCCT (for human cells) TTCATCGTGCTGGGAGCC (for bat cells) or 
TTCATTGTGCTGGGTGCC (for human cells) (FAM)
CATGAAGTGYGACGTBGACATCCG(BHQ1)

*CoV, coronavirus; N, nucleocapsid protein; SARS, severe acute respiratory syndrome.

Main Article

1These authors contributed equally to this article.

Page created: July 17, 2020
Page updated: November 19, 2020
Page reviewed: November 19, 2020
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