Volume 26, Number 3—March 2020
Research
Human Immune Responses to Melioidosis and Cross-Reactivity to Low-Virulence Burkholderia Species, Thailand1
Figure 3

Figure 3. Ex vivo IFN-γ ELISpot responses to Burkholderia pseudomallei, B. thailandensis, and BTCV, Thailand. IFN-γ responses were quantified for acute melioidosis patients (n = 82) and 3 control cohorts: HH, n = 93), diabetic patients (DM, n = 95), and patients with other gram-negative infections (OGNI, n = 42) against whole-cell heat-killed antigens of Burkholderia pseudomallei (BP, red dots), Burkholderia thailandensis (BT, green diamonds), and Burkholderia thailandensis CPS variant (BTCV, blue triangles) are shown. Each symbol represents the average number of SFU per subject. Medians (horizontal lines) and interquartile ranges (error bars) are shown. p values were calculated by using the nonparametric Mann-Whitney test. Horizontal bars at top of figure indicate comparisons across cohorts. BTCV, B. thailandensis CPS variant; CPS, capsular polysaccharide; DM, patients with diabetes mellitus; HH, healthy household contacts of melioidosis case-patients; IFN-γ, interferon-γ; NS, not significant; SFU, spot-forming units.
1Preliminary results from this study were presented at the European Melioidosis Congress, March 19–21, 2018, Oxford, UK.