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Volume 26, Number 6—June 2020
Research

Radical Change in Zoonotic Abilities of Atypical BSE Prion Strains as Evidenced by Crossing of Sheep Species Barrier in Transgenic Mice

Alba Marín-Moreno1, Alvina Huor1, Juan Carlos Espinosa, Jean Yves Douet, Patricia Aguilar-Calvo2, Naima Aron, Juan Píquer, Sévérine Lugan, Patricia Lorenzo, Cecile Tillier, Hervé Cassard, Olivier Andreoletti, and Juan María TorresComments to Author 
Author affiliations: Centro de Investigación en Sanidad Animal, Madrid, Spain (A. Marín-Moreno, J.C. Espinosa, P. Aguilar-Calvo, J. Píquer, P. Lorenzo, J.M. Torres); Interactions Hôte Agent Pathogène–École Nationale Vétérinaire de Toulouse, Toulouse, France (A. Huor, J.Y. Douet, N. Aron, S. Lugan, C. Tiller, H. Cassard, O. Andreoletti)

Main Article

Figure 3

Bovine-PrP transgenic mice challenged with atypical BSEs transmitted into human-PrP transgenic mice before and after adaptation to sheep-PrP sequence in a study of atypical BSE transmission using isolates from different countries in Europe and transgenic mouse models overexpressing human normal brain prion protein. Brain PrPres in TgBo mice inoculated with different atypical BSE either before or after passage into the different transgenic lines. L-BSE biochemical profile (lane 2) changed once pa

Figure 3. Bovine-PrP transgenic mice challenged with atypical BSEs transmitted into human-PrP transgenic mice before and after adaptation to sheep-PrP sequence in a study of atypical BSE transmission using isolates from different countries in Europe and transgenic mouse models overexpressing human normal brain prion protein. Brain PrPres in TgBo mice inoculated with different atypical BSE either before or after passage into the different transgenic lines. L-BSE biochemical profile (lane 2) changed once passaged into TgVRQ (lane 3) and TgARQ (lane 4). L-BSE/TgVRQ produced a PrPres profile resembling the one of C-BSE (lanes 1 and 8). L-BSE propagation into TgARQ produced a 21kDa PrPres profile. H-BSE (lane 5) can still infect back TgBo line once passaged into TgVRQ and adapted to the human PrP sequence (lanes 6 and 7) and produced a 21 kDa PrPres profile. All inoculated animals were analyzed and individual variations in the PrPres profile among them were not found. Lane 1, C-BSE2; lane 2, BSE L2; lane 3, BSE L2/TgVRQ/TgBo; lane 4, BSE L2/TgARQ/TgBo; lane 5, BSE H3; lane 6, BSE H3/TgVRQ/TgMet129/TgBo; lane 7, BSE H3/TgVRQ/TgVal129/TgBo; lane 8, C-BSE2. BSE, bovine spongiform encephalopathy; C-BSE, classical bovine spongiform encephatlopathy; PrP, prion protein; PrPres, proteinase K–resistant prion protein.

Main Article

1These first authors contributed equally to this article.

2Current affiliation: University of California–San Diego, La Jolla, California, USA.

Page created: May 18, 2020
Page updated: May 18, 2020
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