Volume 26, Number 6—June 2020
Research
Radical Change in Zoonotic Abilities of Atypical BSE Prion Strains as Evidenced by Crossing of Sheep Species Barrier in Transgenic Mice
Table 3
Intracerebral inoculation of TgBo with sCJD, L-BSE, and H-BSE isolates after their adaptation (P2) in various hosts in a study of atypical BSE transmission using isolates from different countries in Europe and transgenic mouse models overexpressing human normal brain prion protein*
| Isolate | Mean survival time, days + SD (n/n0)† |
|
|---|---|---|
| P1 | P2 | |
| BSE L2 | 263 + 31 (6/6) | 204 + 4 (6/6) |
| BSE L2→TgVRQ (P2) | 221 + 3 (6/6) | 212 + 3 (6/6) |
| BSE L2→TgARQ (P2) | 240 + 18 (6/6) | 215 + 5 (6/6) |
| BSE L2→TgVRQ (P2)→TgVal129 (P2) | >650 (0/6) | ND |
| BSE H3 | 382 + 74 (6/6) | 262 + 3 (6/6) |
| BSE H3→TgVRQ (P2) | >650 (0/6) | >650 (0/6) |
| BSE H3→TgVRQ (P2)→TgMet129 (P2) | 671, 699,‡ 759‡ (3/6) | 631 + 34 (5/6) |
| BSE H3→TgVRQ (P2)→TgVal129 (P2) | 627,‡ 689‡ (2/6) | 703‡ (1/6) |
| sCJD MM1→TgMet129 (P2) | 750 + 18 (3/6) | ND |
| sCJD MM1→TgVal129 (P2) | 737, 763,‡ 833‡ (3/4) | ND |
| sCJD VV2→TgVal129 (P2) | 833‡ (1/6) | ND |
| vCJD→TgMet129 (P2) | 249 + 2 (6/6) | 236 + 5 (6/6) |
*BSE, bovine spongiform encephalopathy; dpi, days post-inoculation; n/n0, diseased proteinase K–resistant prion protein–positive/inoculated animals; P1, first passage; P2, second passage, sCJD, sporadic Creutzfeldt-Jakob disease; vCJD, variant Creutzfeldt-Jakob disease.
†Survival time is indicated as mean dpi + SD for all mice that scored positive for proteinase K–resistant prion protein.
‡Found dead animals without clinical signs and positive for proteinase K–resistant disease-associated isoform.
1These first authors contributed equally to this article.
2Current affiliation: University of California–San Diego, La Jolla, California, USA.