Volume 26, Number 6—June 2020
Research
Radical Change in Zoonotic Abilities of Atypical BSE Prion Strains as Evidenced by Crossing of Sheep Species Barrier in Transgenic Mice
Table 2
Intracerebral inoculation of TgBo, TgVRQ, and TgARQ mice with atypical BSE isolates to promote adaptation to the sheep-PrP sequence in a study of atypical BSE transmission using isolates from different countries in Europe and transgenic mouse models overexpressing human normal brain prion protein*
Isolate | Mean survival time, d + SD (n/n0)† |
|||||||
---|---|---|---|---|---|---|---|---|
TgBo |
TgVRQ |
TgARQ |
||||||
P1 | P2 | P1 | P2 | P1 | P2 | |||
BSE L2 |
263 + 31 (6/6) |
208 + 21 (6/6) |
438 + 20 (6/6) |
168 + 22 (6/6) |
386‡, 404 (2/6) |
155 + 8 (6/6) |
||
BSE H3 | 382 + 74 (6/6) | 262 + 3 (6/6) | 801 (1/6) | 408 + 44 (6/6) | >700 (0/6) | >700 (0/6) |
*BSE, bovine spongiform encephalopathy; dpi, days post-inoculation; n/n0, diseased proteinase K–resistant prion protein–positive/inoculated animals; P1, first passage; P2, second passage.
†Survival time is indicated as mean dpi + SD for all mice that scored positive for proteinase K–resistant prion protein.
‡Found dead animals without clinical signs and positive for proteinase K–resistant disease-associated isoform.
1These first authors contributed equally to this article.
2Current affiliation: University of California–San Diego, La Jolla, California, USA.