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Volume 26, Number 8—August 2020
Dispatch

Plasma-Derived Extracellular Vesicles as Potential Biomarkers in Heart Transplant Patient with Chronic Chagas Disease

Nuria Cortes-Serra, Maria Tays Mendes, Clara Mazagatos1, Joan Segui-Barber, Cameron C. Ellis, Cristina Ballart, Ana Garcia-Alvarez, Montserrat Gállego, Joaquim Gascon, Igor C. Almeida, María Jesús PinazoComments to Author , and Carmen Fernandez-BecerraComments to Author 
Author affiliations: Barcelona Institute for Global Health (ISGlobal), Universitat de Barcelona, Barcelona, Spain (N. Cortes-Serra, C. Mazagatos, J. Segui-Barber, C. Ballart, M. Gállego, J. Gascon, M.J. Pinazo, C. Fernandez-Becerra); Border Biomedical Research Center, The University of Texas at El Paso, El Paso, Texas USA (M.T. Mendes, C.C. Ellis, I.C. Almeida); Secció de Parasitologia, Departament de Biologia, Sanitat i Medi Ambient, Facultat de Farmàcia i Ciències de l’Alimentació, Universitat de Barcelona, Barcelona, Spain (C. Ballart, M. Gállego); Arrhythmias Unit, Hospital Clinic, University of Barcelona, Barcelona, Spain (A. Garcia-Alvarez); Institut d’Investigació en Ciències de la Salut Germans Trias i Pujol, Badalona, Spain (C. Fernandez-Becerra)

Main Article

Table 2

Trypanosoma cruzi and human proteins identified in plasma-derived EVs from a heart transplant patient with chronic Chagas disease before benznidazole chemotherapy but absent after treatment and in healthy donors

Protein name† UniProt accession no. Unique peptides
ChD Pre-BZN‡ ChD Post-BZN Healthy 1 Healthy 2
T. cruzi
Pyruvate. phosphate dikinase OS = Trypanosoma cruzi 
marinkellei GN = MOQ_000480 PE = 3 SV = 1
 K2MVM1_TRYCR
 2 (0.96)
 0
 0
 0
Homo sapiens
Collagen α-1(VI) chain OS = Homo sapiens GN = COL6A1 
PE = 1 SV = 3 CO6A1_HUMAN 3 (1.44) 0 0 0
Group of Angiopoietin-related protein 6 OS = Homo sapiens 
GN = ANGPTL6 PE = 1 SV = 1+1 ANGL6_HUMAN (+1) 3 (1.44) 0 0 0
sp|PPIA_HUMAN| sp|PPIA_HUMAN| 3 (1.44) 0 0 0
Mannan binding lectin serine protease 2 OS = Homo sapiens GN = MASP2 PE = 1 SV = 4 MASP2_HUMAN 2 (1.92) 0 0 0
Myosin regulatory light chain 12B OS = Homo sapiens GN = MYL12B PE = 1 SV = 2 ML12B_HUMAN 2 (1.92) 0 0 0
Collagen α-2(VI) chain OS = Homo sapiens GN = COL6A2 PE = 1 SV = 4 CO6A2_HUMAN 2 (1.44) 0 0 0
Collectin subfamily member 10 (C-type lectin). isoform CRA_a OS = Homo sapiens GN = COLEC10 PE = 4 SV = 1 tr|A0A024R9J3|A0A024R9J3_HUMAN 2 (1.44) 0 0 0
Group of Coagulation factor XIII A chain OS = Homo sapiens GN = F13A1 PE = 1 SV = 4+2 F13A_HUMAN (+2) 2 (1.44) 0 0 0
Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein. eta polypeptide. isoform CRA_b OS = Homo sapiens GN = YWHAH PE = 3 SV = 1 tr|A0A024R1K7|A0A024R1K7_HUMAN 2 (1.44) 0 0 0
Fibrinogen-like protein 1 OS = Homo sapiens GN = FGL1 PE = 1 SV = 3 FGL1_HUMAN 2 (0.96) 0 0 0
Group of L-lactate dehydrogenase A chain OS = Homo sapiens GN = LDHA PE = 1 SV = 2+1 LDHA_HUMAN (+1) 2 (0.96) 0 0 0
Group of Laminin subunit α-2 OS = Homo sapiens GN = LAMA2 PE = 1 SV = 1+1 A0A087WX80_HUMAN (+1) 2 (0.96) 0 0 0
Group of MHC class I antigen (Fragment) OS = Homo sapiensGN = HLA-A PE = 3 SV = 1+3 tr|D2KZ27|D2KZ27_ HUMAN (+3) 2 (0.96) 0 0 0
Group of Serum amyloid A protein OS = Homo sapiens GN = SAA1 PE = 1 SV = 1+2 E9PQD6_HUMAN (+2) 2 (0.96) 0 0 0
Group of Transforming growth factor β-induced 68kDa isoform 2 (Fragment) OS = Homo sapiens GN = TGFBI PE = 2 SV = 1+1 tr|A0A0S2Z4K6|A0A0S2Z4K6_HUMAN (+1) 2 (0.96) 0 0 0
Heparan sulfate proteoglycan 2 (Perlecan). isoform CRA_bOS = Homo sapiens GN = HSPG2 PE = 4 SV = 1 tr|A0A024RAB6|A0A024RAB6_HUMAN 2 (0.96) 0 0 0
Neurogenic locus notch homologue protein 3 OS = Homo sapiens GN = NOTCH3 PE = 1 SV = 2 NOTC3_HUMAN 2 (0.96) 0 0 0
V1–16 protein (Fragment) OS = Homo sapiens GN = V1–16 PE = 4 SV = 1 tr|Q5NV81|Q5NV81_ HUMAN 2 (2.88) 0 0 0
Rheumatoid factor RF-ET6 (Fragment) OS = Homo sapiens PE = 2 SV = 1 tr|A2J1N5|A2J1N5_ HUMAN 2 (5.29) 0 0 0

*BZN, benznidazole; ChD, Chagas disease.
†All proteins were identified by at least 2 unique peptides. A unique peptide is defined as a peptide found only in >1 protein members of the same protein family of a certain proteome.
‡Normalized total spectrum count values are indicated in parenthesis. Complete mass spectrometry proteomic data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRoteomics IDEntifications Database (PRIDE, https://www.ebi.ac.uk/pride/) partner repository, with the dataset identifier PXD014668 and 10.6019/PXD014668.

Main Article

1Current affiliation: CIBER Epidemiología y Salud Pública (CIBERESP); Centro Nacional de Epidemiología, Instituto de Salud Carlos III, Madrid, Spain.

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Page updated: July 18, 2020
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