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Volume 26, Number 8—August 2020
Dispatch

Plasma-Derived Extracellular Vesicles as Potential Biomarkers in Heart Transplant Patient with Chronic Chagas Disease

Nuria Cortes-Serra, Maria Tays Mendes, Clara Mazagatos1, Joan Segui-Barber, Cameron C. Ellis, Cristina Ballart, Ana Garcia-Alvarez, Montserrat Gállego, Joaquim Gascon, Igor C. Almeida, María Jesús PinazoComments to Author , and Carmen Fernandez-BecerraComments to Author 
Author affiliations: Barcelona Institute for Global Health (ISGlobal), Universitat de Barcelona, Barcelona, Spain (N. Cortes-Serra, C. Mazagatos, J. Segui-Barber, C. Ballart, M. Gállego, J. Gascon, M.J. Pinazo, C. Fernandez-Becerra); Border Biomedical Research Center, The University of Texas at El Paso, El Paso, Texas USA (M.T. Mendes, C.C. Ellis, I.C. Almeida); Secció de Parasitologia, Departament de Biologia, Sanitat i Medi Ambient, Facultat de Farmàcia i Ciències de l’Alimentació, Universitat de Barcelona, Barcelona, Spain (C. Ballart, M. Gállego); Arrhythmias Unit, Hospital Clinic, University of Barcelona, Barcelona, Spain (A. Garcia-Alvarez); Institut d’Investigació en Ciències de la Salut Germans Trias i Pujol, Badalona, Spain (C. Fernandez-Becerra)

Main Article

Figure 1

Isolation and characterization of plasma-derived EVs. A) Schematic diagram of the isolation and characterization of EVs derived from plasma samples. The details of each step are explained in The Study section. B) EVs were characterized by BBA using the classical EV markers CD5L, CD9, and CD63. C) NTA of SEC fractions F7–10. BBA, bead-based assay; EV, extracellular vesicle; LC-MS/MS, liquid chromatography–tandem mass spectrometry; MFI, median fluorescence intensity; NTA, nanoparticle tracking ana

Figure 1. Isolation and characterization of plasma-derived EVs. A) Schematic diagram of the isolation and characterization of EVs derived from plasma samples. The details of each step are explained in The Study section. B) EVs were characterized by BBA using the classical EV markers CD5L, CD9, and CD63. C) NTA of SEC fractions F7–10. BBA, bead-based assay; EV, extracellular vesicle; LC-MS/MS, liquid chromatography–tandem mass spectrometry; MFI, median fluorescence intensity; NTA, nanoparticle tracking analysis; SEC, size-exclusion chromatography.

Main Article

1Current affiliation: CIBER Epidemiología y Salud Pública (CIBERESP); Centro Nacional de Epidemiología, Instituto de Salud Carlos III, Madrid, Spain.

Page created: April 27, 2020
Page updated: July 18, 2020
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