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Volume 27, Number 1—January 2021
Research

Intrafamilial Exposure to SARS-CoV-2 Associated with Cellular Immune Response without Seroconversion, France

Floriane Gallais, Aurélie Velay, Charlotte Nazon, Marie-Josée Wendling, Marialuisa Partisani, Jean Sibilia, Sophie Candon, and Samira Fafi-KremerComments to Author 
Author affiliations: Strasbourg University Hospital, Strasbourg, France (F. Gallais, A. Velay, C. Nazon, M.-J. Wendling, M. Partisani, J. Sibilia, S. Fafi-Kremer); Strasbourg University, Strasbourg (F. Gallais, A. Velay, C. Nazon, M.-J. Wendling, J. Sibilia, S. Fafi-Kremer); Rouen University Hospital, Rouen, France (S. Candon)

Main Article

Figure 3

Frequency of specific T cells directed against spike glycoprotein antigens of the 2 common cold HCoVs 229E and OC43 in study of intrafamilial exposure to SARS-CoV-2, France. A) Index patients (n = 11); B) seronegative partners of index patients (n = 11); C) unexposed healthy controls (n = 10). Spot counts of common cold human coronaviruses-specific T cells were measured by interferon-gamma ELISPOT assay. All experiments were performed in duplicate. Data are shown as means and standard deviations of spot counts of interferon-gamma–producing T cells per 1 million CD3+ cells. T-cell secretion of IFN-γ was determined in response to peptide pools spanning the N-terminal and the C-terminal regions of the spike glycoprotein of HCoV 229E (ES1 and ES2 subpools) and HCoV OC43 (OS1 and OS2 subpools). Each color corresponds to 1 antigen subpool. C, contact; HCoV, human coronavirus; HD, healthy blood donor (control); P, index patient; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2.

Figure 3. Frequency of specific T cells directed against spike glycoprotein antigens of the 2 common cold HCoVs 229E and OC43 in study of intrafamilial exposure to SARS-CoV-2, France. A) Index patients (n = 11); B) seronegative partners of index patients (n = 11); C) unexposed healthy controls (n = 10). Spot counts of common cold human coronaviruses-specific T cells were measured by interferon-gamma ELISPOT assay. All experiments were performed in duplicate. Data are shown as means and standard deviations of spot counts of interferon-gamma–producing T cells per 1 million CD3+ cells. T-cell secretion of IFN-γ was determined in response to peptide pools spanning the N-terminal and the C-terminal regions of the spike glycoprotein of HCoV 229E (ES1 and ES2 subpools) and HCoV OC43 (OS1 and OS2 subpools). Each color corresponds to 1 antigen subpool. C, contact; HCoV, human coronavirus; HD, healthy blood donor (control); P, index patient; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2.

Main Article

Page created: September 29, 2020
Page updated: December 21, 2020
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