Correlation of SARS-CoV-2 Subgenomic RNA with Antigen Detection in Nasal Midturbinate Swab Specimens
Katherine Immergluck
1, Mark D. Gonzalez, Jennifer K. Frediani, Joshua M. Levy, Janet Figueroa, Anna Wood, Beverly B. Rogers, Jared O’Neal, Roger Elias-Marcellin, Allie Suessmith, Julie Sullivan, Raymond F. Schinazi, Ahmed Babiker, Anne Piantadosi, Miriam B. Vos, Greg S. Martin, Wilbur A. Lam, and Jesse J. Waggoner
Author affiliations: Emory University, Atlanta, Georgia, USA (K. Immergluck, M.D. Gonzalez, J.K. Frediani, J.M. Levy, J. Figueroa, A. Wood, B.B. Rogers, J. O’Neal, R. Elias-Marcellin, A. Suessmith, J. Sullivan, R.F. Schinazi, A. Babiker, A. Piantadosi, M.B. Vos, G.S. Martin, W.A. Lam, J.J. Waggoner); The Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta (K. Immergluck, M.D. Gonzalez, J.K. Frediani, J.M. Levy, J. Figueroa, A. Wood, B.B. Rogers, J. O’Neal, A. Suessmith, J. Sullivan, R.F. Schinazi, M.B. Vos, G.S. Martin, W.A. Lam, J.J. Waggoner); Children’s Healthcare of Atlanta, Atlanta (M.D. Gonzalez, M.B. Vos)
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Figure 2
Figure 2. Concordance of SARS-CoV-2 sgRNA with nucleocapsid antigen detection in MT swab specimens, but not NP swab specimens, from study participants in Atlanta, Georgia, USA. sgRNA remains detectable in NP swab specimens for persons who showed negative results for nucleocapsid antigen. Symbols represent MT (blue) and NP (purple) swab specimens for persons with (filled circles) and without (open circles) detectable sgRNA. Horizontal bars indicate means, and error bars indicate SDs. Ct, cycle threshold; MT, nasal midturbinate; NP, nasopharyngeal; N2, nucleocapsid 2; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2; sgRNA, subgenomic RNA.
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