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Volume 27, Number 4—April 2021
Dispatch

Stability of SARS-CoV-2 RNA in Nonsupplemented Saliva

Isabel M. Ott1, Madison S. Strine1, Anne E. Watkins, Maikel Boot, Chaney C. Kalinich, Christina A. Harden, Chantal B.F. Vogels, Arnau Casanovas-Massana, Adam J. Moore, M. Catherine Muenker, Maura Nakahata, Maria Tokuyama, Allison Nelson, John Fournier, Santos Bermejo, Melissa Campbell, Rupak Datta, Charles S. Dela Cruz, Shelli F. Farhadian, Albert I. Ko, Akiko Iwasaki, Nathan D. Grubaugh2Comments to Author , Craig B. Wilen2, Anne L. Wyllie2Comments to Author , and the Yale IMPACT Research team3
Author affiliations: Yale School of Public Health, New Haven, Connecticut, USA (I.M. Ott, A.E. Watkins, C.C. Kalinich, C.A. Harden, C.B.F. Vogels, A. Casanovas-Massana, A.J. Moore, M.C. Muenker, M. Nakahata, A.I. Ko, N.D. Grubaugh, A.L. Wyllie); Yale School of Medicine, New Haven (M.S. Strine, M. Boot, M. Tokuyama, A. Nelson, J. Fournier, S. Bermejo, M. Campbell, R. Datta, C.S. Dela Cruz, S.F. Farhadian, A.I. Ko, A. Iwasaki, C. Wilen); Howard Hughes Medical Institute, New Haven (A. Iwasaki)

Main Article

Figure 2

Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in saliva samples tested for infectious SARS-CoV-2. SARS-CoV-2 N1 detection (Ct values) measured by quantitative reverse transcription PCR for each saliva sample incubated with Vero-E6 cells for 72 hours. The orange diamond depicts the only sample that produced plaque-forming units (titer increase of 3.79 × 104 PFU/mL; purple circles indicate samples that did not produce plaque-forming units by 72 h after inoculation; dashed lines indicate Ct 38 (the cutoff for sample positivity); gray shading indicates Cts below the limit of detection. Ct, cycle threshold.

Figure 2. Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in saliva samples tested for infectious SARS-CoV-2. SARS-CoV-2 N1 detection (Ct values) measured by quantitative reverse transcription PCR for each saliva sample incubated with Vero-E6 cells for 72 hours. The orange diamond depicts the only sample that produced plaque-forming units (titer increase of 3.79 × 104 PFU/mL; purple circles indicate samples that did not produce plaque-forming units by 72 h after inoculation; dashed lines indicate Ct 38 (the cutoff for sample positivity); gray shading indicates Cts below the limit of detection. Ct, cycle threshold.

Main Article

1These first authors contributed equally to this article.

2These senior authors contributed equally to this article.

3Team members team are listed at the end of this article.

Page created: February 09, 2021
Page updated: March 18, 2021
Page reviewed: March 18, 2021
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