Volume 27, Number 4—April 2021
Dispatch
Stability of SARS-CoV-2 RNA in Nonsupplemented Saliva
Isabel M. Ott1, Madison S. Strine1, Anne E. Watkins, Maikel Boot, Chaney C. Kalinich, Christina A. Harden, Chantal B.F. Vogels, Arnau Casanovas-Massana, Adam J. Moore, M. Catherine Muenker, Maura Nakahata, Maria Tokuyama, Allison Nelson, John Fournier, Santos Bermejo, Melissa Campbell, Rupak Datta, Charles S. Dela Cruz, Shelli F. Farhadian, Albert I. Ko, Akiko Iwasaki, Nathan D. Grubaugh2
, Craig B. Wilen2, Anne L. Wyllie2 , and the Yale IMPACT Research team3
, Craig B. Wilen2, Anne L. Wyllie2 , and the Yale IMPACT Research team3
Table
Possible saliva collection devices for severe acute respiratory virus coronavirus 2 RNA testing
| Tube type | Collection | Buffer type | Cost per sample, USD | Manufacturer |
|---|---|---|---|---|
| Oragene•Dx collection device (OGD-510)* |
Funnel |
Ethanol <24%; Tris 1%–5% (host DNA stabilization) |
28.00 |
Genotek, https://www.dnagenotek.com |
| Samplify SD-3000 |
Funnel |
Dry preservative; sodium dodecyl sulfate <1% |
24.00 |
Samplify (URL not available) |
| Saliva collection kit |
Funnel |
Unknown |
22.47 |
IBI Scientific, https://www.ibisci.com |
| SDNA-1000 small tubes* |
Wide-mouth tube |
Ethanol 10%–25%;
Tris 1%–5%; thiocyanic acid:guanidine (1:1)
25%–50%; pH 7.9–8.3 |
17.99 |
Spectrum Solutions, https://spectrumsolution.com |
| Saliva RNA Collection and Preservation Device |
Wide-mouth tube |
Unknown liquid, colorless, odorless |
18 |
Norgen (Biotek), https://norgenbiotek.com |
| Liquid biopsy/spit devices |
Complicated unit (various) |
Unknown |
9–12 each |
Oasis Diagnostics, https://4saliva.com |
| OMNIgene•ORAL saliva collection device (OM-505)* |
Funnel |
Sodium dodecyl sulfate
1%–5%; glycine,N,N′-trans- 1,2-cyclohexanediylbis
[N-(carboxymethyl)-,hydrate
1%–5%; lithium chloride 0.5%–1.5% |
9.50 |
Genotek |
| GeneFix Saliva DNA/RNA Collection |
Funnel |
Unknown liquid, colorless |
9 |
Isohelix, https://isohelix.com |
| DNA/RNA Shield saliva collection kit* |
Wide-mouth tube |
Unknown liquid, colorless,
pH 5.0–7 |
7.25 |
Zymo Research, https://www.zymoresearch.com |
| Saliva collection system |
Small beaker |
Unknown |
Unavailable |
Greiner Bio-One, ttps://www.gbo.com |
| Pedia•SAL Infant/Toddler Salivary Collection |
Soother + collector |
None |
Unavailable |
Oasis Diagnostics |
| Oral swab |
Swab |
None |
1.76 |
Salimetrics, https://salimetrics.com |
| Saliva collection aid + cryovial |
Straw + 2 mL collection vial |
None |
1.36/straw, 0.76/vial |
Salimetrics |
| Urine collection cups |
Wide-mouth cup |
None |
0.47 |
ThermoFisher, https://www.thermofisher.com |
| Sterile tube, large volume | Wide-mouth tube | None | 0.46 (25 mL), 0.38 (5 mL) | Eppendorf, https://www.eppendorf.com |
| Sterile tube, small volume | Narrow-mouth tube | None | 0.16 (2 mL) | ThermoFisher |
*Approved by US Food and Drug Administration Emergency Use Authorization for saliva-based diagnostics.
1These first authors contributed equally to this article.
2These senior authors contributed equally to this article.
3Team members team are listed at the end of this article.
Page created: February 09, 2021
Page updated: March 18, 2021
Page reviewed: March 18, 2021
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