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Volume 27, Number 8—August 2021
Research

Modeling Immune Evasion and Vaccine Limitations by Targeted Nasopharyngeal Bordetella pertussis Inoculation in Mice

Illiassou Hamidou Soumana12, Bodo Linz13, Kalyan K. Dewan1Comments to Author , Demba Sarr, Monica C. Gestal4, Laura K. Howard5, Amanda D. Caulfield, Balázs Rada, and Eric T. Harvill
Author affiliation: University of Georgia, Athens, Georgia, USA

Main Article

Figure 3

Host immune responses to LD and HD Bordetella pertussis inoculation. C57/Bl6 mice received LD of 500 CFU of B. pertussis in 5 µL phosphate-buffered saline (PBS) via nasopharyngeal inoculation or HD of 500,000 of B. pertussis CFU in 50 µL PBS via pneumonic inoculation. Naive control mice were inoculated with 50 µL of PBS. The study was conducted twice. A–D) Enumeration of immune cells in the lungs 14 days postinoculation. E–H) Enumeration of immune cells from nasopharyngeal washes. Error bars indicate SD for 4 biologic replicates HD, high-dose–high volume; LD, low-dose–low-volume; ns, no statistical significance.

Figure 3. Host immune responses to LD and HD Bordetella pertussis inoculation. C57/Bl6 mice received LD of 500 CFU of B. pertussis in 5 µL phosphate-buffered saline (PBS) via nasopharyngeal inoculation or HD of 500,000 of B. pertussis CFU in 50 µL PBS via pneumonic inoculation. Naive control mice were inoculated with 50 µL of PBS. The study was conducted twice. A–D) Enumeration of immune cells in the lungs 14 days postinoculation. E–H) Enumeration of immune cells from nasopharyngeal washes. Error bars indicate SD for 4 biologic replicates HD, high-dose–high volume; LD, low-dose–low-volume; ns, no statistical significance.

Main Article

1These authors contributed equally to this article.

2Current affiliation: University of British Columbia, Vancouver, British Columbia, Canada.

3Current affiliation: Friedrich Alexander University Erlangen-Nuremberg, Erlangen, Germany.

4Current affiliation: Louisiana State University Health Science Center, Shreveport, Louisiana, USA.

5Current affiliation: Augusta University, Augusta, Georgia, USA.

Page created: June 16, 2021
Page updated: July 18, 2021
Page reviewed: July 18, 2021
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