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Volume 27, Number 8—August 2021
Research

Modeling Immune Evasion and Vaccine Limitations by Targeted Nasopharyngeal Bordetella pertussis Inoculation in Mice

Illiassou Hamidou Soumana12, Bodo Linz13, Kalyan K. Dewan1Comments to Author , Demba Sarr, Monica C. Gestal4, Laura K. Howard5, Amanda D. Caulfield, Balázs Rada, and Eric T. Harvill
Author affiliation: University of Georgia, Athens, Georgia, USA

Main Article

Figure 6

Risk for Bordetella pertussis reinfection after experimental nasopharyngeal infection of mice. C57Bl/6J mice were inoculated intranasally with 500 CFU of B. pertussis in 5 µL PBS for nasopharyngeal inoculations (blue squares) or 500,000 CFU in 50 µL for the pneumonic inoculations (red squares). The study was conducted twice. Values are the SD of 4 biologic replicates. A) Number of B. pertussis bacteria in respiratory organs on day 7 after pneumonic challenge. B) B. pertussis IgG titers (log scale) in the serum of mice challenged via PNC or NPL inoculation. Green represents naive mice inoculated with PBS. NPL, nasopharyngeal; PBS, phosphate-buffered saline; PNC, pneumonic.

Figure 6. Risk for Bordetella pertussis reinfection after experimental nasopharyngeal infection of mice. C57Bl/6J mice were inoculated intranasally with 500 CFU of B. pertussis in 5 µL PBS for nasopharyngeal inoculations (blue squares) or 500,000 CFU in 50 µL for the pneumonic inoculations (red squares). The study was conducted twice. Values are the SD of 4 biologic replicates. A) Number of B. pertussis bacteria in respiratory organs on day 7 after pneumonic challenge. B) B. pertussis IgG titers (log scale) in the serum of mice challenged via PNC or NPL inoculation. Green represents naive mice inoculated with PBS. NPL, nasopharyngeal; PBS, phosphate-buffered saline; PNC, pneumonic.

Main Article

1These authors contributed equally to this article.

2Current affiliation: University of British Columbia, Vancouver, British Columbia, Canada.

3Current affiliation: Friedrich Alexander University Erlangen-Nuremberg, Erlangen, Germany.

4Current affiliation: Louisiana State University Health Science Center, Shreveport, Louisiana, USA.

5Current affiliation: Augusta University, Augusta, Georgia, USA.

Page created: June 16, 2021
Page updated: July 18, 2021
Page reviewed: July 18, 2021
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