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Volume 27, Number 8—August 2021
Research

Modeling Immune Evasion and Vaccine Limitations by Targeted Nasopharyngeal Bordetella pertussis Inoculation in Mice

Illiassou Hamidou Soumana12, Bodo Linz13, Kalyan K. Dewan1Comments to Author , Demba Sarr, Monica C. Gestal4, Laura K. Howard5, Amanda D. Caulfield, Balázs Rada, and Eric T. Harvill
Author affiliation: University of Georgia, Athens, Georgia, USA

Main Article

Figure 4

Host cytokine responses to LD nasopharyngeal inoculation and HD pneumonic inoculation of Bordetella pertussis. C57/Bl6 mice received LD of 500 CFU of B. pertussis in 5 µL phosphate-buffered saline (PBS) via nasopharyngeal inoculation or HD of 500,000 of B. pertussis CFU in 50 µL PBS via pneumonic inoculation. Naive control mice were inoculated with 50 µL of PBS. Splenocytes were isolated from mice at day 14 postinoculation. A) IL-17; B) IL-4; and C) IL-10. Error bars indicate SD for 4 biologic replicates; analysis was conducted once. HD, high-dose–high volume; IL, interleukin; LD, low-dose–low-volume.

Figure 4. Host cytokine responses to LD nasopharyngeal inoculation and HD pneumonic inoculation of Bordetella pertussis. C57/Bl6 mice received LD of 500 CFU of B. pertussis in 5 µL phosphate-buffered saline (PBS) via nasopharyngeal inoculation or HD of 500,000 of B. pertussis CFU in 50 µL PBS via pneumonic inoculation. Naive control mice were inoculated with 50 µL of PBS. Splenocytes were isolated from mice at day 14 postinoculation. A) IL-17; B) IL-4; and C) IL-10. Error bars indicate SD for 4 biologic replicates; analysis was conducted once. HD, high-dose–high volume; IL, interleukin; LD, low-dose–low-volume.

Main Article

1These authors contributed equally to this article.

2Current affiliation: University of British Columbia, Vancouver, British Columbia, Canada.

3Current affiliation: Friedrich Alexander University Erlangen-Nuremberg, Erlangen, Germany.

4Current affiliation: Louisiana State University Health Science Center, Shreveport, Louisiana, USA.

5Current affiliation: Augusta University, Augusta, Georgia, USA.

Page created: June 16, 2021
Page updated: July 18, 2021
Page reviewed: July 18, 2021
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