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Volume 28, Number 12—December 2022
Research

Emergence and Evolutionary Response of Vibrio cholerae to Novel Bacteriophage, Democratic Republic of the Congo1

Meer T. Alam2, Carla Mavian2Comments to Author , Taylor K. Paisie, Massimiliano S. Tagliamonte, Melanie N. Cash, Angus Angermeyer, Kimberley D. Seed, Andrew Camilli, Felicien Masanga Maisha, R. Kabangwa Kakongo Senga, Marco Salemi, J. Glenn Morris, and Afsar AliComments to Author 
Author affiliations: University of Florida Emerging Pathogens Institute, Gainesville, Florida, USA (M.T. Alam, C. Mavian, T.K. Paisie, M.S. Tagliamonte, M.N. Cash, F.M. Maisha, M. Salemi, J.G. Morris, Jr., A. Ali); University of Florida College of Medicine, Gainesville (C. Mavian, T.K. Paisie, M.S. Tagliamonte, M.N. Cash, M. Salemi, J.G. Morris, Jr.); University of California, Berkeley, California, USA (A. Angermeyer, K.D. Seed); Chan Zuckerberg Biohub, San Francisco, California, USA (K.D. Seed); Tufts University School of Medicine, Boston, Massachusetts, USA (A. Camilli); Appui Medical Integre aux Activites de Laboratoire (AMI-LABO), Goma, Democratic Republic of the Congo (R.K.K. Senga); University of Goma, Goma (R.K.K. Senga); University of Florida College of Public Health and Health Professions, Gainesville (M.T. Alam, A. Ali)

Main Article

Table

Characteristics of toxigenic Vibrio cholerae O1 strains isolated from the Democratic Republic of the Congo, 2015–2017*

Strain Isolation date Province/location Serotype
 Susceptibility of V. cholerae to ICP1_2017_A_DRC† Mutation in O1 antigen and other genes‡ SRA ID
Ogawa Inaba
AGC-1 2015 Apr 30 North Kivu/Kirotshe – + S – SRR15192533
AGC-2 2015 May 18 Goma/Buhimba – + S – SRR15192532
AGC-3 2015 May 20 Mutwanga – + R rfbD SRR15192521
AGC-4 2015 Mar 07 Goma/Buhimba – + R rfbN SRR15192516
AGC-5 2015 Mar 20 Goma/Buhimba – + S – SRR15192515
AGC-6 2015 Jul 26 Goma/Buhimba – + R rfbV, VC0559 (hypothetical), rplE, phrA, fliD, VC0672 (hypothetical) SRR15192514
AGC-7 2015 Jun 06 Goma/Buhimba – + S – SRR15192513
AGC-8 2015 Aug 06 Goma/Buhimba – + S – SRR15192512
AGC-9 2016 Jun 20 Maniema/Kabambare + – S – SRR15192511
AGC-10 2016 Aug 09 Karisimbi/Hop Millitaire – + R rfbD SRR15192510
AGC-11 2016 May 28 Alimbongo – + R rfbD SRR15192531
AGC-12 2016 Jul 27 South Kivu/Fizi + – S – SRR15192530
AGC-13 2016 Aug 08 Maniema/Kimbilulenge + – S – SRR15192529
AGC-14 2017 May 18 Kirotshe/Rubaya – + S – SRR15192528
AGC-15 2017 May 31 Rutshuru/Hgr – + S – SRR15192527
AGC-16 2017 Jun 10 Rutshuru/Hgr – + S – SRR15192526
AGC-17 2017 Jul 01 Nyiragongo/Turunga – + S – SRR15192525
AGC-18 2017 Jul 03 Goma/Hop.Provincial – + S§ manA SRR15192524
AGC-19 2017 Jul 03 Goma/Hop.Provincial – + S – SRR15192523
AGC-20 2019 Jul 03 Goma/Hop.Provincial – + S – SRR15192522
AGC-21 2017 Jul 06 Karisimbi/Prison centrale – + S – SRR15192520
AGC-22 2017 Jul 14 Karisimbi/Majengo – + S§ manA SRR15192519
AGC-23 2017 Jul 19 Karisimbi/Majengo – + R rfbB SRR15192518
AGC-24 2017 Jul 15 Karisimbi/Majengo – + S rfbU SRR15192517

*R, resistant; S, susceptible; +, positive; –, negative †Susceptibility to a virulent ICP1 phage (ICP1_2017_A_DRC) determined by strains yielding either complete resistance or forming turbid plaques in response to phage infection in plaque assay. The penultimate column indicates which strains had mutations in the O1-antigen biosynthetic complex and in other genes in the chromosome, with the mutated gene designated. AGC-18, AGC-22, and AGC-24 sustained 1, 1, and 18 bp deletion mutations in the indicated gene(s), resulting in a frame shift mutation in that gene, but all other ICP1 phage-resistant isolates sustained >1 missense mutation in the O-antigen biosynthetic gene cluster. ‡As detected by analysis using single-nucleotide polymorphism, insertion/deletion, or both. §Plaques were turbid as described elsewhere (29).

Main Article

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Main Article

1Previously presented at Epidemics—8th International Conference on Infectious Diseases Dynamics [online], November 30–December 3, 2021.

2These authors contributed equally to this article.

Page created: October 18, 2022
Page updated: November 21, 2022
Page reviewed: November 21, 2022
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