Figure 2. Scatter plot demonstrating detection of Heartland virus (HRTV) by real-time, quantitative reverse transcription PCR. Data were not normally distributed and are presented as medians with interquartile ranges. Fed female carcasses, egg pools, and larvae pools were screened for viral RNA. Egg pools and larvae pools were composed of 50 eggs or larvae per pool. Limit of detection was ≈10 FFU equivalents/μg RNA. FFU, focus-forming units.