Volume 28, Number 3—March 2022
Dispatch
Transovarial Transmission of Heartland Virus by Invasive Asian Longhorned Ticks under Laboratory Conditions
Wilson R. Raney, Josiah B. Perry, and Meghan E. Hermance
Table 2
Rate of detection of HRTV RNA by qRT-PCR and infectious HRTV by FFA in fed Haemaphysalis longicornis adult tick carcasses, tick eggs, and tick larvae*
| Procedure | Real-time qRT-PCR detection of HRTV RNA, no. positive/no. tested (%) |
FFA titration of HRTV, larvae pools‡ | ||
|---|---|---|---|---|
| Fed adult carcasses | Egg pools† | Larvae pools† | ||
| Medium injected | 0/5 (0) | 0/15 (0) | 0/20 (0) | 0/5 (0) |
| HRTV-injected | 5/5 (100) | 15/15 (100) | 20/20 (100) | 5/5 (100) |
*FFA, focus-forming assay; HRTV, Heartland virus; qRT-PCR, quantitative reverse transcription PCR. † Egg and larvae pools contained 50 eggs or larvae/pool. ‡Larvae pools contained 150 larvae/pool.
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