Volume 28, Number 3—March 2022
Dispatch
Transovarial Transmission of Heartland Virus by Invasive Asian Longhorned Ticks under Laboratory Conditions
Table 1
Procedure | Real-time qRT-PCR detection of HRTV RNA, no. positive/no. tested (%) |
FFA titration of HRTV, whole tick | ||
---|---|---|---|---|
Salivary glands | Midgut | Carcass | ||
Medium injected | 0/15 (0) | 0/15 (0) | 0/15 (0) | 0/9 (0) |
HRTV-injected 14 dpi | 4/8 (50) | 8/8 (100) | 8/8 (100) | 5/5 (100) |
HRTV-injected 21 dpi | 7/8 (88) | 8/8 (100) | 8/8 (100) | 5/5 (100) |
HRTV-injected 28 dpi | 6/8 (75) | 8/8 (100) | 8/8 (100) | 5/5 (100) |
HRTV-injected 40 dpi | 3/6 (50) | 6/6 (100) | 6/6 (100) | 4/5 (80) |
*dpi, days postinjection; FFA, focus-forming assay; HRTV, Heartland virus; qRT-PCR, quantitative reverse transcription PCR.
Page created: December 08, 2021
Page updated: February 21, 2022
Page reviewed: February 21, 2022
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.