Figure 4. Phylogenomics of novel HeV variant from horse in Australia. A) Maximum-likelihood phylogeny of paramyxoviruses using complete L protein sequences. Gray shading indicates henipaviruses, and red text indicates the novel HeV variant, which groups with the prototypic HeV. Bootstrap support values as proportions of 500 replicates are shown at nodes; values <0.7 are hidden. Scale bar indicates substitutions per site. B) Enlarged gray area from panel A shows branch lengths for the henipavirus clade. The branch leading back to the common ancestor of all known HeVs and the novel HeV variant does not exceed 0.03; thus, they are considered variants of the same species. C) Maximum-likelihood phylogeny of partial N and P where deep branch lengths have been collapsed for visualization only to demonstrate that the variant is well outside the known diversity of HeV. Scale bar indicates substitutions per site. D) Nucleotide genomic similarity of the variant compared with the prototypic HeV strain. V,W, and C indicate variably transcribed nonstructural proteins. F, fusion; G, glycoprotein; HeV, Hendra virus; L, paramyxovirus polymerase; M, matrix protein; N, nucleoprotein; P, phosphoprotein.